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The research and development of functional foods to the food industry into a new production of functional food, functional food base material that preparation of physiologically active substances. Functional oligosaccharides have unique physiological functions, in particular, can promote the proliferation of intestinal bifidobacteria beneficial to intestinal health became an important functional food base material, has attracted worldwide attention. XOS is composed of D-xylose unit through β-1, 4 - glycosidic bond oligosaccharides, xylobiose and xylotriose, it is extremely difficult to be digested and absorbed the residual rate in the intestine, with a very good proliferation of Bifidobacterium, the choice to use higher than that of other functional oligosaccharides. Corn is one of China's three major food crops, the annual corn cob production is extremely impressive, except a small amount of initial processing, most of the farm fuel is burned, and that is a waste of resources and environmental pollution. Therefore, to carry out the corncob as raw materials, of XOS production of theory, research and production technology is essential. Beginning in 2007, the research group members relying Jilin Province Department of Education funded project (No. 415) \extraction and qualitative and quantitative research is research important content of the paper is carried out in this context. Currently, the detection of its main component of XOS studies have been completed. The main purpose of this project is to design and optimize the extraction and purification process route, and qualitative and quantitative study of its components. The research work includes: brief introduction XOS significance, the development of the history of its research status. 2, the corn cob total sugar extraction and purification of. Using hot water extraction test analysis of the corn cob total sugar extraction process route blend of performance considerations determine the optimal process routes: extraction temperature 90 ℃, solid-liquid ratio 1:25 (g / mL), Baptist extraction time 2.5h, once extraction, 80% alcohol precipitation operation, under this condition, the extraction rate of 13.18%. Freezing and thawing the graded impurity removal, Sevage law in addition to protein, active carbon purification test corncob total sugar. The single factor test to determine carbon decolorization conditions: temperature 70 ℃, 4% of the amount of active carbon decoloration time 60 min, activated carbon for sugar decolorization rate of 78.04 percent, sugar loss rate of 16.61%. UV spectral results show that nucleic acids and proteins purified corncob total sugar. 3 corncob XOS preparation. The SephadexG-25 column chromatography preparation, corn cob total sugar and part of the acid hydrolysis of sugar samples were obtained after column chromatography operation Xylo component A and component B of xylooligosaccharides. Column chromatography conditions: Eluent: 0.1 mol / L NaCl, solution; sample concentration: 4%; the sample volume: Column volume = 1:35; eluent flow rate: 0.17 column volumes / h. Sugar desalting treatment by the ion exchange resin, effectively reduce the salt content in the sugar solution, desalting rate was 61.33%. Corncob XOS qualitative and quantitative analysis. HPLC chromatogram of component A and component B, the main component A xylobiose, containing a small amount of xylotriose, xylobiose purity of up to 91.27%, the yield was 3.91%; component B mainly xylobiose purity of 95.62%, the yield was 6.84%; may contain a small amount of xylotriose lower purity, yield is only 0.22%. By component B MS spectra analysis shows contains xylobiose, xylotriose the tetrasaccharide wood and wood pentasaccharide.
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