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Real-time detection of heavy metal ions on human health and environmental monitoring is important. Recently, the use of biological macromolecules nucleic acid as a biological sensor to detect heavy metal ions in the environment has become a research hotspot of the people . However, in the the biosensor development process , there is the problem of interference of other ions . In this paper, for each Pb of 2 , the Hg 2 < / sup > detection , to study the impact of different ions . Characterized by circular dichroism experiments Pb 2 , Zn 2 and the Mg 2 < / sup > on Pb 2 -dependent DNAzyme conformation , results showed that , Zn 2 Pb 2 -dependent deoxyribozyme conformation there is a certain the Zn 2 reduce chiral deoxyribozyme . < / Sup > detected in the the Hg 2 experiments using fluorescently labeled DNA ( D- ODN - F ) , by the quenching of the fluorescence intensity detected in the aqueous solution of the Hg 2 < / sup > . Experimental study of different temperature and the concentration of D - ODN - F interference in the Hg 2 < / sup > detection process . The results show that the temperature of 20 ° C , is conducive detected the Hg 2 < / sup> , at the same temperature , low concentrations of D - ODN - F is not conducive to the detected the Hg 2 < / sup> when the D - ODN - F concentration increased to 60 nm , the interference of other ions the Hg 2 < / sup > detection can be ignored. Circular dichroism and non-denaturing polyacrylamide gel electrophoresis experiment results show that the other metal ions ( the Mg 2 < / sup > , the Pb 2 and of Cu 2 < / sup > ) ODN conformation a certain extent.
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