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The Effects of Intravitreous Bevacizumab on Angiogenesis in a BN Rat Model of Laser-Induced Choroidal Neovascularization.

Author: YuZuo
Tutor: LiLei；ShenYing；ZhangYongJin
School: Fudan University
Course: Ophthalmology
Keywords: Choroidal neovascularization Antibodies, monoclonal Angiogenesis inhibitors Indocyanine green angiography Rats
CLC: R773.4
Type: Master's thesis
Year: 2008
Downloads: 39
Quote: 1
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Abstract


Choroidal neovascularization in exudative age-related macular degeneration, pathologic myopia variety of diseases such as severe visual impairment. Clinical studies show that intravitreal injection of VEGF-A full-length antibody bevacizumab can induce a variety of reasons led to the CNV and idiopathic CNV subsided macular edema. Clinical applications, however, the the single vitreous cavity of the majority of patients given bevacizumab visual acuity improved in not a long time to maintain. On bevacizumab CNV inhibition in vivo study due to the CNV model the defects could not be exact reaction bevacizumab efficacy of CNV. Therefore, we intend to take advantage of the laser-induced the BN rat CNV model, by observing bevacizumab (Avastin TM ) of its generation and development, to understand the characteristics of bevacizumab for CNV expectations for better application it provide preliminary experimental evidence for the treatment of human CNV. The BN rat choroidal neovascularization induced by the first part of the semiconductor laser research purposes: To investigate the semiconductor laser to create Brown Norway (brown Norway, BN) rat choroidal neovascularization (choroidal neovascularization, CNV) the feasibility of the model, and to lay the foundation for the CNV Prevention Research. Methods: Male BN rats 24, each rat randomly take an experimental eye, the other eye as a control eyes. Semiconductor laser (wavelength 561nm) around the rat eye optic photocoagulation, laser power, spot diameter and exposure time were 120Mw, 100μm and 0.1s, each vision condensate 8:00. Animals were sacrificed after photocoagulation 3,7,14,21,28,35 and the 56d line fundus fluorescein angiography (fundus fluoresceinangiography, FFA) and indocyanine green angiography (indocyanine green angiography, ICGA) inspection, removal of the eye prepare specimens for histopathological observation. Results: ICGA shows CNV after laser photocoagulation 3d, the 14d when fully developed. FFA examination showed CNV cylindrical a discoid high fluorescence, leakage intensity stable at 14 to 28d. Photocoagulation after 14d, visible under the light microscope subretinal CNV formation, its central thickness of 14 to 28d remained stable. Conclusion: semiconductor laser-induced the BN rat CNV model, model short time, to maintain a long time. Objective: To study the use of the second part of Bevacizumab the BN rat CNV generate and developmental effects of laser-induced the BN rat CNV model to explore the inhibitory effect of intravitreal injection of bevacizumab on CNV development. Methods: 12 male BN rats, each rat were randomly take one as an experimental eye, the other eye as a control eyes. First with the semiconductor laser photocoagulation experimental eye retina, and then randomly divided into the bevacizumab group and control group. The Bevacizumab group (6) given in the of photocoagulation immediate intravitreal the 2μl Avastin TM , while the control group (n = 6) give 2μl balanced salt solution. 7d, 14d and 21d line FFA and ICGA observed after laser photocoagulation. 21d take experimental eye after photocoagulation specimens HE staining and VEGF immunohistochemical staining. Results: 7d, ICGA after photocoagulation bevacizumab group and the control group both CNV generated, but this time the FFA fluorescence intensity ratings of the bevacizumab group than in the control group, the difference was significant. However, the group rated subsequently elevated to 21d but remained in the control group after photocoagulation. 21d, the the Bevacizumab group of CNV average central thickness after laser photocoagulation lower than the control group, the difference was statistically significant, and Immunohistochemistry showed VEGF negative. Conclusion: in the BN rat the CNV formation of early intravitreal injection of bevacizumab can inhibit the development of CNV from morphological and leakage function. But does not completely prevent the formation of the CNV, also not long maintain its effect of suppressing the leakage function. Bevacizumab in combination with other treatment methods may be able to improve the treatment efficiency of the CNV.

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CLC: > Medicine, health > Ophthalmology > Eye pigment layer ( uvea ) disease > Choroidal disease
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