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Tea in China is the earliest as drug applications, and later became a daily must-drink. The main component of tea - tea polyphenols (polyphenols of tea, PPT) has antioxidant, anti-tumor, anti-viral, anti-bacterial and anti-radiation effect. In ischemia-reperfusion injury in the generation of oxygen free radicals and vascular endothelial growth factor (Vascular endothelial growth factor, VEGF) secretion plays an extremely important role. In ischemia-reperfusion (Ischemia-Reperfusion IR) injury, the generation of oxygen free radicals and the secretion of VEGF plays an extremely important role. Ischemia, hypoxia-induced changes in cell function, increased cell permeability to produce large amounts of oxygen free radicals after reperfusion, direct damage tissue; VEGF increased secretion of vascular endothelial injury [1]. In the gastrointestinal ischemia-reperfusion aspects, there are a large number of domestic and foreign literature reports, but tea polyphenols in literature not much of a direct protective effect and mechanisms of gastrointestinal gastrointestinal ischemia-reperfusion injury, few reports. This study was to rat plasma through the detection of tea polyphenols intervention peroxide dismutase (superoxide dismutase, SOD), malondialdehyde (malondialdehyde, MDA), the gastric vascular endothelial cells connection to observe tea polyphenols antioxidant Clear radicals role of the gastrointestinal vascular endothelial protection, further testing the mucosal vascular endothelial VEGF and CD34 with gastric vascular endothelial growth close to the change, as well as by the observation of the TPs treatment cases, ischemic reperfusion of the gastric mucosal capillary vascular endothelial ultrastructural changes, investigate the protective effect of tea polyphenols on gastric ischemia-reperfusion. Methods: 35 SD rats were randomly divided into 7 groups (n = 5), were established: control group; sham control group; surgery control group; 20mg/kg PPT group; 40mg/kg PPT group; 80mg/kg PPT group; 160mg/kg PPT group. Establish 12h specimens from gastric ischemia-reperfusion rat model, modeling success after 30min reperfusion, while giving the appropriate dose of the drug, respectively, at 1 h after reperfusion, 2h, 6h, 12h specimens from plasma samples at reperfusion body mucosal tissue samples. ① application colorimetric assay was measured before and after administration of each group of experimental rats, its plasma MDA, SOD levels change; the ② immune fluorescence staining, synchronization observed connection between each experimental group reflect the endothelial cells of gastric mucosa VEGF and CD34 changes; ③ transmission electron microscopy methods, synchronous observation capillary endothelial ultrastructure changes in the gastric mucosa of each experimental group. Results: ① SOD content of the corresponding point in time for each dose group was significantly higher than the surgical control group, sham operation group and blank control group, 40mg/kg group the most significant. ② 20mg/kg, 40mg/kg, 80mg/kg group corresponding time points MDA content was significantly lower than the surgical control group, sham operation group and blank control group, 80mg/kg group the most significant 160mg/kg group is slightly lower than surgery control group, higher than the sham control group and blank control group. ③ administered group gastric mucosa capillary endothelial CD34 expression level was significantly higher than the surgical control group, slightly lower than the sham control group and blank control group. ④ administered group gastric mucosa capillary endothelial VEGF expression levels were significantly lower than the surgical control group, slightly higher than the sham-operated group and normal control group. ⑤ to tea polyphenols treatment, the ultrastructure of capillary endothelial cells of gastric mucosa injury can be significantly reduced. Conclusion: TP due to ischemia and reperfusion causes damage to the gastric mucosa has a protective effect, its main pathway by scavenging oxygen free radicals and improve the repair of the vascular endothelium in the gastric mucosa.
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