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Purpose in recent years found that statin lipid effects on bone metabolism also affected. The past, some studies observed statins promote bone formation, and whether the drug inhibits bone resorption reported rarely. The experimental design of the experimental study of the diabetic rats with simvastatin in order to observe whether the drug can prevent the occurrence and development of osteoporosis in diabetic rats by inhibiting bone resorption, and explore its possible mechanism, and thus for diabetes and bone The prevention and treatment of osteoporosis provide experimental basis. Methods Male Wistar rats (Experimental Animal Center of Anhui Medical Research Institute), 2-month-old, 200 ± 20 g body weight, the measured tail blood sugar before the experiment were normal, were randomly divided into three groups: group A, normal control group ; B group, diabetic control group; C group, simvastatin treatment group, n = 8. Diabetes modeling the C group the simvastatin 20mg/kg.d (Merck Limited), dissolved in distilled water ig A, B group was fed normal saline. Detect the 2nd week, blood sugar, in two weeks, eight weeks using metabolic cages to collect 12-hour urine, accurate metering mix specimens from 5 ml of urine type I collagen amino measured by enzyme-linked immunosorbent assay telopeptide weekend 8 rats were killed through the femoral artery blood amino-terminal peptide of type I collagen using enzyme-linked immunosorbent assay measured blood, bone mineral density measured by total body BMD. After carefully removed the rat femur around lower limbs muscle tissue, determination of bilateral femur bone mineral density, and specimens from three groups of rats femur bone samples were observed by light microscopy microscope change. The results of blood sugar (1) among the groups compare: 2,8 weeks, B, C group were significantly higher than in group A (P lt; 0.01), between B and C group group without statistical differences (P gt; 0.05). (2) the groups inter urine NTX: the second weekend in group B, C group was significantly higher than that in group A (P lt; 0.01); compare urine NTX group B rose 8 weekend weekend group, while Group C significantly decreased after simvastatin treatment NTX than before treatment, the difference was statistically significant (P lt; 0.05), but still higher than the A group. (3) the groups between blood NTX compare: B group, C group was significantly higher than that in group A (P lt; 0.01) compared with group B, group C after simvastatin treatment significantly decreased. (4) groups between bone mineral density in comparison: B, C group than in the control group was significantly lower reduction C group after simvastatin treatment, bone mineral density than that in group B, the difference was statistically significant P lt; 0.05, but with the A group, there is still a large gap, the difference was statistically significant (P lt; 0.01). (5) of the light microscope, the bone tissue pathological changes compared with group A, B group light microscopic findings (1:400) trabecular bone was significantly thinner, sparse, fracture, during which more adipose tissue. Osteoporosis pathological changes improved feeding simvastatin group C compared with group A, there are still significant osteoporosis performance. Conclusion: Simvastatin bone tissue of diabetic rats clear protective effect and its mechanism may inhibit bone resorption, the exact mechanism is worth further study.
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