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Objective To study the new blood Xuefuzhuyutang endothelial function and myocardial fibrosis in hypertensive rats, which illustrate the relationship between the hypertensive rat endothelial function and the prothrombotic state to improve hypertension myocardial fibrosis. 40 10-week-old SPF the clean male rats (Qingdao Institute for Drug Control) were randomly divided into five groups: model group, captopril group, TCM group, sham group, the control group, n = 8, The average weight of 180 ± 17g. Establishment of abdominal aortic constriction induced rat model of renal hypertension, decoction of traditional Chinese medicine group new blood Xuefuzhuyutang for successful modeling 3ml / d orally (drug concentration: crude drug 25g/3ml), 1 times / d; the captopril group captopril 100mg/kg 3ml diluted with double distilled water gavage, 1 / d; daily distilled water 3mL gavage, model group, sham operation group and the control group. Animals in each group were gavage fasting in the morning, 8 weeks, weighing once a week, weight-adjusted dose. Intervention after 8 weeks, the rats were weighed, blood of the rats were sacrificed and hearts were removed rapidly excised, the removal of the great vessels, atria, and right ventricle along the atrioventricular junction, sufficiently washed with PBS, dry filter paper, weighing the left ventricle (including the interventricular septum) wet weight as left ventricular mass index (LVMI), left ventricular weight to body weight ratio. ABC-ELISA method using double antibody Central heart, the quantitative determination of the level of plasma von Willebrand factor (vWF) and endothelin (ET), the level of plasma D-dimer (DD) using the turbidimetric method to immunohistochemical chemical staining method to observe changes in myocardial collagen type III and type I collagen. Results of rat plasma vWF, FT and DD TCM group and captopril group of plasma vWF and ET decreased compared with the model group, a significant difference (P <0.01), the model group than in the captopril group, TCM group, sham group, blank control group significantly increased (P <0.01) after Chinese Medicines medication plasma of vWF, ET and DD with sham surgery group, blank control group, each group of relatively non-significant with difference (P> 0.05). Captopril plasma DD TCM group, the comparison between the sham group, blank control group, each group increased (P <0.05). Rats systolic blood pressure compared the Chinese Medicines blood pressure compared with the model group generally declined, compared to a significant difference (P <0.05). Chinese medicine group and captopril group blood pressure decreased, but not as good as captopril group, blood pressure decreased significantly Medicines Board, Chinese Medicines blood pressure captopril group, sham operation group, blank control group, elevated blood pressure (P <0.05). Model group with the captopril group, sham group, blank control group comparison, blood pressure was significantly higher, there is a significant difference (P <0.01); groups in left ventricular mass index (LVWI) compared with the captopril group model group decreased more significant difference (P <0.01). TCM group compared with the model group decreased more significant difference (P <0.05), and Chinese medicine group and captopril group showed no significant difference (P> 0.05). Collagen type I: type I collagen captopril group and Chinese medicine group CVF and PVCA compared with the model group decreased (P <0.05), significantly higher than the model group of collagen type I CVF and PVCA sham group and blank group (P <0.01), collagen type III: captopril group and Chinese medicine group, collagen type III CVF PVCA compared with the model group was significantly lower (P <0.05), collagen type III CVF and PVCA model group and the sham group and blank group significantly increased (P <0.01). The conclusion of Xuefuzhuyu soup can improve endothelial function and hypertension hypertensive rats prothrombotic state and significantly better than the model group (untreated group), and new blood Xuefuzhuyutang is improve and prevent hypertensive rat myocardial fibers the aspect of a certain role, and captopril group showed no significant difference, Description The new blood Xuefuzhuyutang prevents the development of myocardial fibrosis in hypertensive rats and reveal of new blood Xuefuzhuyutang vascular endothelial functional improvement and its intervention in the intrinsic link between myocardial fibrosis, and provide a theoretical basis for progress in preventing myocardial fibrosis and hypertension treatment Chinese medicine research and development.
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