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In this study, morphological markers and RAPD marker method the genetic diversity of 22 representative thin-skinned melon germplasm resources , the results are as follows : 22 germplasm botany and biology traits observed through the system , screening special use value germplasm material . Standardization method with a standard deviation of phenotypic traits using SPSS software , Num13 threshold , the germplasm is divided into five categories , draw the dendrogram of genetic relationship , the figure does not reflect the cotton of melon varieties of groups in the traditional classification system . Improved SDS method to extract DNA to good effect . RAPD reaction , the DNA template RNA amplification results had no effect ; macromolecules such as proteins, polysaccharides , and SDS, chloroform , isopropyl alcohol , and other small molecules that interfere with the PCR results ; appropriate concentration of template DNA wide ; optimized RADP The conditions were as follows : 20ul system containing dNTP 200mmol / L, Taq enzyme 1U buffer 2UL templates 50ng ultrapure water 13.5ul , random primers, 5 pmol . Reaction program : 96 ° C denaturation 5min adding Taq polymerase , and then amplified conditions were as follows : denaturation at 94 ° C for 1 min , 36 ° C renaturation 1min extension at 72 ° C for 2min , 40 cycles . 126 polymorphic bands were amplified with 12 random primers to obtain the selected 22 Genetic distances range from 0.084 to 0.230 , the difference is smaller . Nei index method , the genetic similarity of 0.865 for the threshold , which is divided into six categories . The results with the results of morphological markers .
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