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Objective: To explore the the psoriatic keratinocyte cell protein tyrosine kinase activity; explore psoriatic keratinocytes cells, the protein tyrosine kinase in the role of Zhuhuang Granule mitogen-activated protein kinase activation. Methods: 40 patients with psoriasis clinical completely randomly divided into the psoriasis group, 10 people the group pretreated group of Zhuhuang Granule, the role of Zhuhuang Granule time the group, Shiraia granules role concentration group, and incorporate healthy volunteers 10, whichever is the skin tissues, keratinocyte cells in culture, the use of immunohistochemical identification of keratinocyte cell type, the use of flow cytometry protein tyrosinase expression, compare psoriasis patients and normal horny forming the difference between the state of tyrosinase phosphorylation of cellular proteins. And the application Shiraia granules II No. extract was role of keratinocytes in psoriasis patients formed cells and adding different concentration gradient (1,10,100 IU / ml) the role of 5 minutes and the time gradient (1,2,5,15 minutes) Western blot observed silk mitogen-activated protein kinase activation. Results: (1) a single-factor analysis of variance results see three groups of F = 77.99, P lt; 0.01, significantly significant. (2) compared with the normal control group, increased expression rate of the the psoriasis group protein tyrosine kinase (p lt; 0.01); the extract processing horny Zhuhuang Granule formation of cells with psoriasis group protein casein histidine kinase expression decreased (P lt; 0.01); the group expression Shiraia granules is higher than the normal group (P lt; 0.01). (3) 50ul Shiraia granules II No. extract solution for 1 minute, 2 minutes, 5 minutes, 15 minutes visible keratinocytes cell protein phosphorylation levels were gradually decreased; 20ul, 50ul, 100ul Shiraia granules II No. extract keratinocytes for five minutes protein phosphorylation levels also was gradually decreased, showing the dependence of the concentration and time. Conclusion: (1) psoriatic keratinocytes cell protein tyrosine kinase activity were significantly higher than those in healthy control group, so the protein tyrosine kinase activity exception must keratinocyte cell proliferation and differentiation exception occurs, thereby affecting keratinocytes occurred; (2) Shiraia granules role in psoriatic keratinocytes forming cells, inhibition of mitogen activated protein kinase activation, keratinocytes can be suppressed the proliferation of the cells, and the degree of anti-proliferative effect in and its concentration was positively correlated.
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