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Expression of Hypoxia Inducible Factor-1alpha and Its Correlation with Lymphangiogenesis in Lung Cancer
Author: ZhouSiJing
Tutor: LiuJian
School: Huazhong University of Science and Technology
Course: Geriatrics
Keywords: Lung cancer Lymphatic microvessel density Hypoxia-inducible factor -1α Vascular endothelial growth factor -C Transforming growth factor -α Lymph node metastasis
CLC: R734.2
Type: Master's thesis
Year: 2010
Downloads: 19
Quote: 0
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Abstract
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Objective: To study lung cancer lymph node metastasis and hypoxia -inducible factor -1α (HIF-1α) relationship , and the mechanism of action of the latter may . Methods : Immunohistochemistry was used to detect 72 cases of cancer tissues , lung cancer and 20 cases of normal lung tissue hypoxia inducible factor - 1α ( HIF - 1α) , transforming growth factor -α (TGF-α) and vascular endothelial growth factor -C (VEGF-C) expression levels using D2-40 immunohistochemical method marked lung tissues ( including lung tissue , peritumoral tissue and normal lung tissue ) in the lymphatic and determination of lung tissue micro - lymphatic density (LMVD), correlation analysis , and three factor expression with the surrounding tissues , lymphatic microvessel density and cancer organizations lymphatic microvessel density and clinicopathological features of the relationship between the expression levels of the three factors statistical comparison . Results: D2-40 staining showed micro- lymphatic (LMV) mainly distributed in the surrounding tissues of the lung tissue , normal lung tissue and lung cancer tissue compared to few lymphatic vessels , lymphatic vessels in cancer tissues often be squeezed and deformed into the pipe for non-functional . A positive correlation between micro - lymphatic vessel density (LMVD) , level of lymph node metastasis of lung cancer with the surrounding tissues . HIF - 1α, TGF-α and VEGF-C protein expression levels in normal lung tissue was significantly lower than the lung tissue (both P lt ; 0.05) ; HIF -1α expression levels of TGF - alpha expression levels of the surrounding tissues LMVD and the expression of VEGF-C levels were positively correlated (r = 0.396,0.444 and 0.452 , both P lt ; were positively correlated (r = 0.05 ) , the expression levels of VEGF - C and TGF-α expression levels and cancer organizations LMVD respectively. 0.357 and 0.523 , all P lt; 0.05) . Conclusion: lung cancer lymph node metastasis in the lung tissue , HIF-1α expression significantly correlated . HIF-1α by upregulating the expression levels of TGF-α and VEGF-C in the lung tissue , and thus participate in the process of lymph node metastasis of lung cancer .
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CLC: > Medicine, health > Oncology > Respiratory system tumors > Lung tumors
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