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Objective To study the chromosomal abnormalities , the SRY gene mutation and Y chromosome AZF gene microdeletion genetic cause of male infertility . The detection oligozoospermia infertility patients blood and sperm genome AZF microdeletions case . Methods chromosome G-banding karyotype analysis of 87 cases of male infertility patients , the use of PCR technology to detect the SRY gene in patients with mutations in the Y chromosome AZF gene microdeletion . In our study, the Y chromosome AZFa , AZFb , AZFc , AZFd area of five STS sites oligospermia , azoospermia patients and 17 normal fertile men , 57 patients with primary molecular detection of Y chromosome microdeletions . 5 pairs of primers selected for the the the AZFa area sY86 the by AZFb the sY127 and the SY134 , the AZFc district sY254, AZFd area sY152 . 1 , 57 specimens be tested by PCR could be detected by specific fragment of the sex-determining gene (Sex-determining region Y, SRY) , show that the detection of DNA template the AZF gene detection amplification system effectively meet the requirements . 2,87 cases of male infertility patients found chromosomal abnormalities in 25 cases ( 28.74 % ) ; XX male reversal ( 1.15 % ) ; AZF microdeletions in 4 cases ( 4.60% ) . The total genetic abnormality detection rate of 34.49% . 3,17 healthy fertile men were no AZF microdeletions , blank and female specimens , no amplified bands . Conclusion 1 , the chromosomal abnormality is cause reproductive abnormalities an important factor . For infertile patients , the use of cytogenetic techniques for karyotype analysis is very important. Normal male chromosome infertility patients can do to further the Y chromosome microdeletion screening in order to identify the etiology of patients . Y chromosome microdeletions in AZF region is an important factor leading to impaired spermatogenesis . Carried out in the clinical detection of AZF microdeletions an important role in the diagnosis of azoospermia and severe oligozoospermia . 3 , the Y chromosome microdeletions through vertical genetic mutation to male offspring , so the primary oligozoospermia and azoospermia , before carrying out artificial insemination or intracytoplasmic sperm injection , related to genetic testing , to prevent genes to the next generation , improve the rate of eugenics has important clinical significance . 4 , chromosomal abnormalities and Y chromosome AZF microdeletions are important genetic cause of male infertility etiology . Of this group , blood, semen genome detection result is the same.
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