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Objective To study the level of pigment epithelium-derived factor (PEDF) the relationship between the levels in the serum of patients with type 2 diabetes mellitus (T2DM) and urinary albumin excretion rate. Explore the pigment epithelium-derived factor in the development of diabetic nephropathy, provide clues and basis for the early diagnosis and treatment of diabetic nephropathy. Methodological study of 120 cases. Which the healthy control group of 30 patients met the inclusion criteria Qingdao Municipal Hospital of healthy subjects (15 men, 15 women) from June 2009 to December 2009, 90 cases of patients with type 2 diabetes, in Qingdao Municipal Hospital of Endocrinology hospitalized patients (44 male, 46 female), divided into of T2DM normal albuminuria group (30 cases), the T2DM microalbuminuria group (30 cases), T2DM with macroalbuminuria group (30 cases), blood pressure (SBP / DBP) was measured to calculate body mass index (BMI), blood was measured fasting plasma glucose (FPG), fasting C-peptide (FCP), cholesterol (CHO), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL), glycosylated hemoglobin (HbA1c) and specimens from 24h urine detection of urinary albumin excretion rate (UAER), using the method of enzyme-linked immunosorbent assay (ELISA) were measured in all selected serum PEDF level. Results ① healthy control group, T2DM normal albuminuria group, T2DM microalbuminuria group, T2DM macroalbuminuria group group, age, gender, there was no significant sex difference (P gt; 0.05), from the healthy control group, T2DM normal proteinuria group of T2DM microalbuminuria group to T2DM macroalbuminuria serum PEDF levels in turn gradually increased (4.86 ± 1.22μg/ml, 6.59 ± 1.29μg/ml, 7.93 ± 1.84μg/ml, 10.17 ± 2.27μg / ml), among the three groups showed a significant difference (P lt; 0.05). ② The Pearson correlation analysis show PEDF and UAER in (r = 0.482, P lt; 0.01), SBP (r = 0.377, P lt; 0.01), DBP (r = 0.331, P lt; 0.01), BMI (r = 0.377, P lt; 0.01), FPG (r = 0.552, P lt; 0.01), HbA1c (r = 0.581, P lt; 0.01), CHO (r = 0.478, P lt; 0.01), TG (r = 0.371, P lt; 0.01), LDL (r = 0.418, P lt; 0.01) were positively correlated with FCP (r = -0.565, P lt; 0.01), HDL (r = -0.321, P lt; 0.01) was negatively correlated with age, Gender had no significant correlation (P gt; 0.05). (3) Multiple linear stepwise regression analysis HbAlc (p = 0.261, P lt; 0.01), CHO (β = 0.242, P lt; 0.05), the UAER in (β = 0.175, P lt; 0.01), HDL (β = -0.191 , P lt; 0.01), BMI (β = 0.182, P lt; 0.01) serum the PEDF level of the independent impact factor. Conclusions Serum PEDF level was significantly higher in the patients with T2DM, and gradually increased with the severity of the patients with albuminuria. HbAlc, CHO, UAER, HDL, BMI is an independent serum the PEDF level of the impact factor. Serum PEDF levels may be elevated in the occurrence and development process of the DN play a some compensatory protection role. Serum PEDF levels may be a sensitive indicator forecast to DN and progress.
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