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Objective: Through observation of Yang Xin Tang Zhongjun drug (ginseng, astragalus) of human umbilical vein endothelial cell proliferation rate impact, from the cellular level to explore its role in prevention and treatment of unstable angina mechanism to provide an objective basis for future clinical applications. Methods: Rats were randomly divided into the Shenqi group, blank group. Blood after 1 week of continuous administration, the serum was separated, and formulated into Shenqi containing serum concentration was 2%, 4%, 6% of the culture broth of the culture broth and blank serum. Human umbilical vein endothelial cells were seeded in culture plates and divided into five groups. 2% of the the Shenqi group, the 4% Shenqi group, the 6% Shenqi group, blank serum group, model group, respectively. The Shenqi group were added to the corresponding concentration of Shenqi culture medium containing serum, blank serum added blank serum-free medium, model group joined the culture medium of human umbilical vein endothelial cells. 2 4 h of culture, with H2 O 2 of vascular endothelial cell model approximation model of atherosclerosis. Using electron microscopy of cell ultrastructure changes detected by MTT OD values ??and calculated the rate of cell proliferation. Results: 1. Observation results show that compared with the model group and blank serum cell contraction and round cell gap significantly widened. Each Shenqi group as Shenqi with increase in the concentration of serum, the cells more into the film adherent growth form of the closer flat polygonal cells nearer clearance. MTT colorimetric assay results show that, compared with the blank serum group, model group OD value is significantly lower, the difference was statistically significance (P lt; 0 0 1); compared with the model group, 2% Shenqi group OD value without significantly increased, the difference was not statistically significance (P gt; 0 0 5) 4% Shenqi group OD value increased, the difference was statistically significance (P lt; 0 0 5), 6% Participation the stilbene group OD value significantly higher, the difference was statistically significance (P lt; 0 0 1); each the Shenqi group group comparison, 6% of the Shenqi group OD value is higher than the other two groups, the difference was statistically significant ( P lt; 0. 0 1). 3. Calculate the rate of cell proliferation results showed that, compared with the model group, 2% of the the Shenqi group proliferation rate without significantly increased, the difference was not statistically significance (P gt; 0 0 5), the proliferation rate of 4% Shenqi group liters high, the difference was statistically significant (P lt; 0 0 5), a significant increase in the proliferation rate of 6% Shenqi group, the difference was statistically significance (P lt; 0 0 1). Conclusion: Shenqi containing serum prevention H 2 O 2 due to the role of human umbilical vein endothelial cell damage, which can promote the proliferation of endothelial cells, may participate Astragalus compatibility through its qi role, rousing, to improve vascular endothelial dysfunction, thereby improving the exception of the vascular endothelial cells, to prevent damage to the endothelial cells play a role in the prevention and treatment of unstable angina.
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