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Experimental study of endothelial progenitor cell mobilization and coronary heart disease risk factors

Author: ZhaoWenYan
Tutor: XiaoChuanShi；QiuLing
School: Shanxi Medical
Course: Cardiovascular within science
Keywords: Fenofibrate endothelial progenitor cells (EPC) granulocyte colony-stimulating factor (G-CSF) atherosclerosis (As) diabetes mellitus (DM)
CLC: R541.4
Type: Master's thesis
Year: 2005
Downloads: 87
Quote: 0
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Abstract


PartⅠ Effects of fenofibrate on the number of endothelial progenitor cells from peripheral blood in rabbit Objective To investigate whether fenofibrate have influence on endothelial progenitor cells (EPC) from peripheral blood contrast to atorvastatin group. Methods 32 male New Zealand white rabbits were divided into fenofibrate group one(50 ㎎/㎏/d), fenofibrate group two(25 ㎎/㎏/d)and control group, there were 8 rabbits in each groups. Before treatment and 1.2.3.4w, peripheral blood was collected. After 7 days cultured, EPC were characterized as double positive for DiLDL-uptake and lectin binding by direct fluorescent staining. After 3 days cultured, the number of EPC (PE -CD34/ FITC-CD133 dual-stained positive cells) was surveyed by Flow Cytometry Analysis . Results Before treatment, the number of EPC was low; After a week treatment of atorvastatin, the number of EPC in atorvastatin was higher than before (≈7-fold, P<0.01), and kept significant higher in 3w (≈20-fold, P<0.001). The number of EPC in fenofibrate group one and two was low throughout the 4-week study period P>0.05. Conclusion fenofibrate (50 ㎎/㎏/d, 25 ㎎/㎏/d) could not mobilize EPC . PartⅡ Effects of atherosclerosis and granulocyte colony-stimulating factor on the number of endothelial progenitor cells from peripheral blood Objective To investigate whether atherosclerosis (As) and granulocyte colony-stimulating factor (G-CSF) have influence on endothelial progenitor cells (EPC) from peripheral blood. Methods 32 male New Zealand white rabbits were divided into G group(Recombinant Human Granulocyte Colony Stimulating Factor Injection rhG-CSF 50μg /d), G+As group (rhG-CSF 50μg/d, high cholesterol diet), As group (high cholesterol diet) and control group, there were 8 rabbits in each groups. Before treatment and 1.4.8.12w, peripheral blood was collected. After 7 days cultured, EPC were characterized as double positive for DiLDL-uptake and lectin binding by direct fluorescent staining. After 3 days cultured, the number of EPC (PE -CD34/ FITC-CD133 dual-stained positive cells) was surveyed by Flow Cytometry Analysis . At 12th week,serum NO and lipids were measured and square of the aortic plaque were researched. Results Before treatment, the number of EPC was low; After a week treatment of G-CSF, the number of EPC in G group and G+As group was fast higher than before (≈13-fold, P<0.001), and kept persistence significant higher in G group in 12w (1.4.8.12W compared each other P>0.05);After fed on high cholesterol diet, the number of EPC in G+As group decreased gradually. At 4th and 8th week, compared with control group, respectively P<0.001, P<0.001;At 12th week, compared with control group P=0.326. The number of EPC in control group had kept low in the whole process. The number of EPC in As group was lower than control group, but P>0.05. After fed on high cholesterol diet, there were aortic plaque in G+As group and As group, but the square of the aortic plaque was significant difference, P<0.01. Conclusion As could impair endothelial cells and reduce the number of EPC. G-CSF could mobilize EPC, improve the number of EPC in peripheral blood, protect blood vessel and prevent As. Part Ⅲ Effects of diabetes mellitus and granulocyte colony-stimulating factor on the number of endothelial progenitor cells from peripheral blood Objective To investigate whether diabetes mellitus (DM) and granulocyte colony-stimulating factor (G-CSF) have influence on endothelial progenitor cells (EPC) from peripheral blood. Methods 32 male New Zealand white rabbits were divided into G group(rhG-CSF 50μg /d), G+DM group (rhG-CSF 50μg/d, DM model), DM group(DM model)and control group, there were 8 rabbits in each groups. Before treatment and 1.2.3.4w, peripheral blood was collected. After 7 days cultured, EPC were characterized as double positive for DiLDL-uptake and lectin binding by direct fluorescent staining. After 3 days cultured, the number of EPC (PE-CD34/ FITC-CD133 dual-stained positive cells) was surveyed by Flow Cytometry Analysis. At 4th week, seru

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CLC: > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Heart disease > Coronary arteries ( atherosclerosis ),heart disease (CHD)
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