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The large number of human proteins, complex interactions, so the results of any physiological, pathological changes, or the influence of drugs are closely related to the protein component and the number of changes. The protein is not only to provide a basis for the study of life sciences, clarify and overcome many disease mechanisms can provide theoretical basis and resolve. Nicotinamide adenine dinucleotide phosphate (coenzyme II) is a naturally occurring coenzyme all vivo, some important cofactor of the enzyme reaction, particularly in the energy transfer in the metabolic activity of the organism and the maintenance of intracellular the redox balance plays a very important role. Coenzyme II loss when an exception occurs ATP generation dysfunction, resulting in the destruction or loss of cellular antioxidant function, will eventually lead to cell death. Heparin by mast cells secrete a wide range of biological functions in the human body and is naturally present in the blood, both in vitro and in vivo to delay or prevent blood coagulation. As a classic anti-clotting drugs, heparin in clinical applications has reached 60 years, is still the choice for cardiovascular and thrombotic patients anticoagulants, heparin and its derivatives can also be used to adjust blood lipids, anti-thrombotic, improving hemorheology, anti-inflammatory, anti-allergic and immune regulation, is one of the important biochemical drugs. Therefore, the study of the interaction of drugs and biological macromolecules and its analytical applications for life sciences research, looking for drug target molecule, novel drug design and the analysis and diagnosis of clinical medicine has a very important significance. This paper in order to establish a new method to detect the actual samples of biological macromolecules, molecular spectroscopy (fluorescence, UV) as a means of research, to explore the lanthanide ions, the mechanism of action of the drug molecules and biological macromolecules, and use of fluorometry analysis of proteins, coenzyme Ⅱ and heparin sensitive, simple method. Is divided into three chapters. Chapter terbium ions - quinolones fluorescent probe, non-radiative energy transfer theory to study the interaction of human serum proteins and its analytical applications. The quinolones with α-carbonyl carboxylic acid structure, is ideal ligands for terbium ion, the addition of human serum proteins terbium ions - quinolones complex system, the characteristic fluorescence of terbium ions significantly enhanced, according to this nature, establishing new method for the determination of human serum albumin, and also discusses the reaction mechanism, and obtaining a combination of the two constants, and successfully used for the determination of the sample. Chapter II, terbium ion - quinolones as a fluorescent probe to study the interaction with heparin its
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