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Research on the Expression of Cycline Messenger RNA in Colonic Carcinoma and the Relation between Cycline and Proliferation Activeness of Cells

Author: JinCuiXiang
Tutor: XuChangQing
School: Shandong University
Course: Internal Medicine
Keywords: colonic carcinoma CyclinE proliferating cell nuclear antigen (PCNA) in-situ hybridization immunohistochemisLry
CLC: R735.35
Type: Master's thesis
Year: 2005
Downloads: 43
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Objective: CyclinE is a proto-oncogene identified in recent years. It promotes cell cycle through promoting its transformation from G1 to S stage, having the effect of adjusting cell proliferation. Proliferating cell nuclear antigen (PCNA) is a polypeptide only synthesized and expressed in proliferating cells, extensively expressed in cell cycle S stage. As a good index for evaluating cell proliferation state, PCNA can reflect the metabolism of tumor cell and synthesizing state of DNA and RNA, the proliferation activeness of which has relation with differentiation, infiltration, metastasis, relapse and prognosis of tumor cello Through detecting the expression of CyclinE and PCNA in colonic carcinoma organization, this research is to study the relation of CyclinE and PCNA with colonic carcinoma biological character, the correlation between CyclinE and PCNA, and their clinic meaning.Methods: Expression of CyclinE mRNA in the organism of 40 cases of colonic carcinoma with various stages, 10 cases of colonic adenoma, 10 cases of normal colonic mucosa was detected by in-situ hybridization method. Expression of CyclinE protein and PCNA in the above organism were examined by S-P immunohistochemistry method.Results: (1) Using immunohistochemistry method, CyclinE protein is not found in normal colonic mucosa. But the expression of it can be found in colonic adenoma and colonic carcinoma, with the positive ratio of 10.0% and 57.5% respectively.The difference between adenoma group and normal group has no statistical meaning (P>0. 05) , and the expression ratio of CyclinE in colonic carcinoma group is obviouslyhigher than in adenoma group and normal group( P<0. 05). (2) By checking the expression of CyclinE mRNA in differernt colonic tissues using technique of in-situ hybridization, it is found that CyclinE mRNA has no expression in 10 cases of normal colonic mucosa;There are 2 cases of CyclinE mRNA positive expression in 10 cases of colonic adenoma, with a positive ratio of 20.0%;In 40 cases of colonic carcinoma, 25 cases have CyclinE mRNA positive expression with different extent, with a ratio of 62. 5%. Expression of CyclinE mRNA in colonic carcinoma obviously increases compared with adenoma group and normal group (P <0. 05) , but the difference between adenoma group and normal group is not notable (P>0.05) .(3) CyclinE mRNA and CyclinE protein have close relation with colonic carcinoma’s biological character. CyclinE mRNA expression has correlation with invasive depth and lymph-node-metastasis (P< 0. 05) . Expression of CyclinE mRNA has no correlation with the age and sex of patient, position and diameter of tumour, extent of histology differentiation (P>0.05) .Difference between group of liver-transfer and non-liver-transfer has no statistical meaning (P >0.05) .This may be due to the relatively few cases of 1 iver-trans for in test. But the positive ratio has a tendency to be higher than group of non-liver-transfer. Expression of CyclinE protein in colonic carcinoma is basicly consistent with the expression of CyclinE mRNA. It is related with the extent of infiltration and lymph-node-metastasis of colonic carcinoma (P<0.05) , and has no relation with patient’ s age and sex, position and diameter of tumour and extent of histology differentiation(P>0. 05). But the positive expression ratio of CyclinE protein in liver-transfer group is higher than m non-liver-transfer group(P<0. 05). (4) Expression of PCNA in different colonic organism was detected using immunohistochemistry method, and the result shows that PCNA labelling index(PCNA LI) has a tendency of increasing (6. 93 + 3. 01 — 24. 91 + 4. 40 — 50. 09+11. 15) in process of "normal mucosa-adenoma-carcinoma" transformation.Obvious statist-ical difference exists among various groups ( P<0. 01) , and expressi on of PCNA in adenoma and carcinoma increases notably.Expression of PCNA has no relation with patient’ s age and sex, position and diameter of tumour (P>0. 05) . But expression of PCNA has notable difference among different extents of differentiation, different depths of infiltration , lymph-node metastasis and non-lymph-node-metastasis (P< 0.05). But expression of PCNA has no correlation with liver-transfer (P>0.05) . This may also be due to relatively few cases of liver-transfer in test. (5)In colonic carcinoma, expression of CyclinE mRNA has close relation with PCNA. PCNA LI is obviously higher in CyclinH mRNA positive colonic carcinoma than in CyclinE mRNA negative colonic carcinoma (P<0.05).Similarly, PCNA LI is also obviously higher in CyclinE protein positive colonic carcinoma than in CyclinE protein negative colonic carcinoma (P<0.05) .Conclusions: (1) CyclinE mRNA and CyclinE protein may have an important effect in the process of colonic carcinoma occurrence and advance. (2) The high expression of CyclinE in colonic carcinoma organism and the consistency of its rerecording level expression with protein level expression suggest that CyclinE could be a target point in colonic carcinoma diagnose and treatment. (3) Expression of PCNA displays obvious tendency of increasing in the process of "normal mucosa- adenoma-carcinoma" transformation, and has close correlation with differentiation extent, infiltration depth and metastasis. It shows that PCNA participates in occurrence and advance of colonic carcinoma and high PCNA may be one of signs indicating bad prognosis. PCNA could be taken as an important index for judging malignancy degree of colonic carcinoma, guiding clinic treatment and improving patient prognosis. (4) CyclinE mRNA and CyclinE protein expression in colonic carcinoma has close correlation with PCNA. It shows that the two may have effect in coordination in colonic carcinoma ocurrence and suggests that CyclinE may be taken as another index of cell prolife-ration of colonic carcinoma.

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