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Detection of GA733 mRNA by RT-PCR in Exfoliated Cells of Pleural and Peritoneal Effusions and Its Clinical Values

Author: CaoFeng
Tutor: ZhangHe
School: Jiangxi Medical College
Course: Internal scientific digest
Keywords: GA733-2 mRNA GA733-1 mRNA RT-PCR Pleural effusion Ascites Exfoliated cells
CLC: R446.9
Type: Master's thesis
Year: 2005
Downloads: 80
Quote: 0
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Abstract


Purpose chest / ascites is a common clinical disease in recent years with the rise in tumor incidence rate of malignant pleural / ascites rising. Correctly judge the the chest / benign and malignant ascites nature, for the patient's diagnosis, treatment and prognosis have important clinical value. The diagnosis of malignant pleural / ascites rely mainly on cytology, but the low rate of positive identification of metastatic cancer cells with reactive hyperplasia of mesothelial cells also often difficult to find the ideal new methods of differential diagnosis has been People are concerned about the research topic. GA733 encoding cancer-associated antigen gene family, which is widely expressed in epithelial cells and epithelial tumors. 80% of human tumors of epithelial origin, more than 90% of malignant pleural / ascites Department of epithelial malignant chest / due to peritoneal metastasis. Does not exist in the epithelial cells of benign chest / ascites, detection Chest / ascites epithelial markers become the basic starting point for people to find malignant pleural / ascites diagnostic methods. To this end, the study by RT-PCR method to detect chest / ascites in epithelial marker the GA733 mRNA expression status, their diagnosis of malignant pleural / ascites, in order to establish a valuable new diagnostic methods. Method Chest / ascites specimens collected 60 cases of fresh, exfoliated cells collected after conventional centrifugal preparation of cell smears, the routine Papanicolaou cytology diagnosis, detailed collection of information and access to medical records, determine specimens clinical diagnosis. Were divided into malignant group, according to the clinical diagnosis of benign disease of unknown etiology group, divided into benign group and malignant group and suspicious malignant group cytology results. Precipitation to partially exfoliated cells as materials used for RT-PCR to detect GA733-1 and GA733-2 mRNA specific process, including the extraction of total RNA was reverse transcribed into cDNA, and use it as a template were amplified by PCR, 800% polypropylene gel electrophoresis, silver staining techniques. GA733-1 and GA733-2 mRNA positive rate, calculate the different groups as well as the diagnosis of malignant pleural / ascites sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic rate. The results of 60 patients with thoracic / ascites specimens, 36 patients diagnosed as malignant, 13 patients diagnosed with benign disease and 11 cases of unknown etiology. Cytology diagnosis of malignant effusion in 26 cases, and benign effusion in 28 cases, six cases of suspicious malignant effusion. In 36 patients with malignant tumors of the chest / ascites specimens, GA733-2 mRNA-positive detection rate

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