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Objective: Severe acute pancreatitis (severe acute pancreatitis, SAP) is the critical illness of the digestive system, high mortality, serious harm to human health. SAP pathogenesis is very complex, the date, the exact mechanism is not yet fully clear, there is no specific treatment means. Therefore, how to effectively control the SAP as the challenges faced by today's medical staff. To solve this problem, it is extremely important to start from the pathogenesis of SAP. The study showed that the nuclear factor-κB (nuclear factor-kappa B, NF-κB) to regulate the release of many inflammatory mediators play an important role in the SAP. At home and abroad in recent years, studies have shown that: peroxisome proliferator-activated receptor γ (Peroxisome proliferator-activated receptor-γ, PPARγ) to participate in the body's inflammatory reaction, its activation can lead to lower NF-kappaB activity and reduce the production of inflammatory mediators . In this study, 5% sodium taurocholate retrograde cholangiopancreatography pressurized injection preparation of rat model of SAP was observed pancreatic gross and histological changes, serum and ascites amylase, and pancreatic tissue NF-kappaB, ICAM-1 protein expression while observing the PPARgamma agonist pioglitazone pretreatment NF-kappaB and ICAM-1 protein expression changes, to investigate the pathogenesis of SAP PPARγ agonists provide a theoretical basis and experimental basis for clinical AP. Methods: 54 healthy male Sprague-Dawley rats, weighing 160-200g, were randomly divided into sham operation group (C group), severe acute pancreatitis group (A) and pioglitazone preconditioning group (group I), n = 18 only. Ketamine hydrochloride anesthetized by intraperitoneal injection retrograde cholangiopancreatography pressurized injection of 5% sodium taurocholate (0.1 ml/100 g) SAP model. I rats 2h preoperative intra-abdominal injection of pioglitazone (2mg/100g). Abdominal aortic phlebotomy were periods of 3, 6, 12 h after the rats were sacrificed (each n = 6), and the abdominal aortic blood and ascites amylase test; pancreas tissue, divided into two parts: the first part was immediately placed fixed in 10% neutral buffered formaldehyde solution (10 ml / L), used for immunohistochemistry and HE staining; put in the second part-80oC refrigerator, to determine pancreatic tissue containing amount of water. Immunohistochemistry two-step detection of pancreatic tissue NF-kappaB and ICAM-1 expression, simultaneous pancreas gross pathology, histological scores and pancreas tissue water content determination; Hughes standard pancreatic pathologic score, histological score based on Kusske standards; pioglitazone pretreatment and observe the changes in the indicators.
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