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Studies on the Molecular Identification of the Cool-season Cultivars of Lentinula Edodes

Author: GongLiJuan
Tutor: LiuShuYan
School: Jilin Agricultural University
Course: Plant Pathology
Keywords: Mushrooms ITS RAPD ISSR Cultivation Properties
CLC: S646.12
Type: Master's thesis
Year: 2005
Downloads: 184
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Abstract


In this study of 32 widely used cool-season mushroom cultivars and two strains of wild mushrooms, the application of ITS sequence comparison between RAPD technology, ISSR molecular markers and culture traits comparative analysis of the genetic correlation between them, The results show that:. ITS region sequence analysis showed that all strains of cultivated mushrooms sequence homology to 98.49%, of which 868, Shanghai farmers, of Qin 19 and Hong 66, as well as wild 11 Hing Branch 20,867 241 - 4 identical sequence. Whether these strains are synonyms to be further confirmed. Instead, China 939,939 L939 similar names, but differences exist five bases ITS sequences. All cultivated strains ITS sequences from GenBank search abroad ITS sequences of the wild-type strain system analysis results found that strains of wild mushrooms and cultivated mushrooms strains belonging to the two branches, cultivated strains of wild strains Genetic differences between cultivated strains of great genetic similarity and genetic relationship of the strains asked and geographical. This further implies that the low genetic diversity of 32 cultivated strains. 9 primer on the analysis of the genomic DNA of the tested strains screened from 86 RAPD random primers amplified a total of 113 with, 94 of which were polymorphic polymorphism with the ratio of 83.19% . The average number of the single primer amplified DNA fragment 13, the molecular weight of the fragment size in the range of 200 ~ 2500bp. Amplified strain using primers A04 868 7 band, the fragment size is between 400 ~ 2300bp. Comprehensive analysis of nine polymorphic DNA fingerprinting, the mushrooms tested 34 strains between both genetic differences, can separate all strains zone to thereby confirmed the RAPD technique can be rapid, sensitive, and accurate for the detection and identification of edible fungi. From genetic similarity coefficients and clustering results indicate that there is a significant genetic differences between wild fruiting bodies and cultivars; most cultivated strains together, the genetic similarity coefficient is mainly concentrated in the 0.50 to 0.70, indicating that the mushroom cultivation strains The genetic background is very single. Strain China 939,939 and L939 together similar kinship may be synonyms. Polymorphism of the strains tested primers screened from 100 ISSR primers amplified to produce a total of 98 DNA bands, 89.80% polymorphic. As primers 812 amplified all strains were obtained with 13. Cluster analysis showed that 34 shiitake strains have genetic differences, but the genetic correlation high. Show that The ISSR amplification fingerprinting effectively distinguish between different strains of genotype to provide fast and effective technical means and the scientific basis for the identification of mushrooms cultivated strains. Strain China 939,939 and L939 together similar kinship may be synonyms. This is consistent with the RAPD results. The 4 by strains cultivated traits test, 32 strains were found some differences in mycelial growth rate, mycelium growing colony morphology and primordium time. Such as flat-panel test, the strain Cr20 slow growth, while Hong 26 fast growth. Treadmill exercise test and trial bottle gardening, mycelial growth rate consistent that in the flat-panel test

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CLC: > Agricultural Sciences > Gardening > Vegetable gardening > Fungi ( mushroom ) > Fold Agarics > Mushrooms ( champignon )
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