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The Theoretical Analysis on the Experiment that the Atomic Force Microscope Interacts with Bilayer Lipid Membrane

Author: LiuYanHui
Tutor: HuLin；OuYangZhongCan
School: Guizhou University
Course: Theoretical Physics
Keywords: atomic force microscope biomembrane bilayer lipid membrane double electric layers Poisson-Bolzmann theory reciprocal theorem lipid domain fluid and mosaic model
CLC: Q73
Type: Master's thesis
Year: 2006
Downloads: 155
Quote: 1
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Abstract


Biological membranes mainly consist of lipids steroids and proteins, as the simplified model of biological membrane, lipid bilayers not only serve as a host matrix for proteins but also exhibit unique electrical and mechanical properties, this paper reviews the liquid crystal biomembrane model and introduces the latest theory about biological membrane: the microdomain in the cell membrane, the single molecule technology on investigating membrane, the theory about atomic force imaging, then introduces the basic theory that we will utilize in the next chapter.but mainly analyzes the experiment that the atomic force microscope interacts with lipid bilayrer, interprets the experimental phenomena. Then we will discuss the following problems.The first, in salt solution, double electric layers exist on thesurface of bilayer lipid membrane. With the decreasing of the ion strength,the atomic force microscope can feel the fore in a longer distance from the surface, which is determined by the Debye lengthSecond, how the strength of the buffer solution affects the stability of bilayer lipid membrane?Third, simulate the linear part of the force spectrum and give the force expression of linear and nonlinear part separately.

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