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PURPOSE: To isolate and culture dental pulp stem cells from human deciduous teeth, then to analyze their biological characteristics and potential to be induced to osteoblasts. The purpose is to find a kind of seed cells with strong proliferation ability and good bone formation performance for bone tissue engineering, which is a novel treatment of bone loss. The experiment is also offer experimental and theoretic basic for the treatment of clinical bone loss.METHODS: Dental pulp stem cells from deciduous teeth were separated with enzyme digestion,then isolated and purified by limited dilution. Tested the cells clone forming rate, mensurated cells growth curve by counting the number of cells, did HE staining and immunhistochemistry staining of Vimentin,STRO-1 and CD44. Then induced the cells to osteoblasts, did HE staining ,AKP staining, Von Kossa staining,Van Gieson staining,and immunhistochemistry staining of osteocalcin.RESULTS: Obtained Dental pulp stem cells from deciduous teeth by enzyme digestion and limited dilution. They could magnify themselves for 20 passages in vitro. The cells clone forming rate was 11-24 /103cells, and cells doubling time was 39.77. Immunhistochemistry staining of dental stem cells from deciduous incisor showed that Vimentin and STRO-1 were positive,and immunofluorescent staining showed CD44 was positive. Induced the cells to osteoblasts , which elicited a biological and morphologic characteristics similar to those of osteoblasts.CONCLUSION: Obtained dental pulp stem cells from deciduous teeth and induced them to osteoblasts successfully.
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