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Background and purpose of ovarian epithelial cancer is one of the common three malignant tumors of the female reproductive system, often non-specific clinical symptoms early, patient visits have been mostly in the late mortality rate ranks first in gynecological malignancies, and 5-year survival rate hovered at 30% to 40%. CA 125 is the only auxiliary sensitive indicators confirm the diagnosis, and 80% of epithelial ovarian cancer in patients with serum CA 125 levels detected, but the level only used in clinical remission and deterioration of the situation in the monitoring of patients with ovarian cancer treatment, is still the lack of relatively specific biological markers for the early diagnosis and prognosis. Therefore, to find ovarian cancer early diagnosis, condition monitoring and prognosis of new indicators to improve the survival rate of patients with ovarian cancer, ovarian cancer research vital topic. Ubiquitin (Ubiquitin, Ub) and its dependent non-lysosomal pathway in tumorigenesis and development process plays an important role, it is highly conserved peptide present in all eukaryotic tissues, free molecules or ubiquitin conjugating the form of the protein (or protein ubiquitination). Ubiquitin is widely distributed in various tissues, a component is involved in abnormal protein translation modification, its main role is involved in protein degradation. Function ubiquitin ligase system - specific ATP-dependent enzyme catalyzed cell protein ubiquitination, and thus the participation of the two major lysosomal and non-lysosomal system ubiquitination cell protein was degraded. Ubiquitin - proteasome pathway that barriers will affect cell cycle regulation, and to strengthen the role of some oncogenes can make the reduced role of the tumor suppressor gene, thereby promoting tumorigenesis. Thus, the cell protein ubiquitin for many cell activity process is critical, including the selective degradation of intracellular proteins and cell stress response of a variety of noxious stimuli and cell injury protection reaction. However, Ub exists in ovarian cancer, abnormal expression of the expression changes can influence the incidence of ovarian cancer development and prognosis is not very clear. Runaway tumor development and malignant transformation, and cell cycle are closely related. Cyclin B1 (CyclinB1) is a classic division cycle protein involved in the regulation of cell cycle checkpoint, and to promote G 2 / M phase transition and accelerated cell cycle progression. Date, mammalian cells found six subtypes of B-type cyclins known as called of CyclinB1 of CyclinB1 occurred mainly in the S phase, and during this period began to express, in G 2 / M when you reach the peak, then quickly ubiquitination and degradation (Ubation), and its over-expression of the cell cycle G 2 / M conversion time is shortened, causing the cells to become cancerous. By immunohistochemical SP assay Ub and CyclinB 1 protein expression in epithelial ovarian cancer in the study, to investigate the expression of epithelial ovarian cancer clinical pathological factors, prognosis for ovarian cancer clinical diagnosis, condition monitoring, prognosis and treatment of the experimental data and the theoretical basis of the science. Materials and Methods 1 collected in our hospital from January 2004 to December 2007 the gynecological inpatient surgical resection of ovarian epithelial tumor paraffin-embedded tissue specimens of 70 patients and normal ovarian tissue specimens of 20 cases were not all specimens preoperative radiotherapy, chemotherapy and other treatments. Epithelial ovarian malignant tissue samples of 50 cases, 9 cases, including 36 cases of serous adenocarcinoma, mucinous cystadenocarcinoma, endometrioid carcinoma 5 cases; benign ovarian tumor specimens of 20 cases, including serous cystadenoma 16 cases, 4 cases of mucinous cystadenoma. 2 immunohistochemistry SP method detected 20 cases of normal ovarian tissue, 20 cases of benign ovarian epithelial tumors and 50 patients with epithelial ovarian cancer tissues Ub and CyclinB1, expression, combined with epithelial ovarian cancer histological type, pathological grade, clinical stage and lymph node metastasis were analyzed, and to study the correlation between the two indicators. 3 Statistical analysis: SPSS13.0 statistical package for data analysis, the expression of of Ub and CyclinB1 protein and its relationship with clinicopathological features using x 2 test, Fisher's exact method, using Spearman rank correlation analysis of both correlation. Α = 0.05 as the level of inspection. Results The positive expression rate of 1 Ub protein in epithelial ovarian cancer, benign ovarian tumors and normal ovarian tissue were 34% (17/50), 10% (2/20), 5% (1/20) of CyclinB1 the The positive expression rate of 42% (21/50), 15% (3/20), 5% (1/20), both positive expression in ovarian cancer were significantly higher than those in normal ovarian tissue and benign ovarian tumors , the difference was statistically significant (P <0.05). 2 epithelial ovarian cancer, Ub expression with histological grade, clinical stage, lymph node metastasis (P <0.05); histological type was no significant correlation (P> 0.05). III, Ⅳ of ovarian cancer tissue the Ub protein expression was significantly higher than Ⅰ, Ⅱ stage, the difference was statistically significant (x 2 = 4.529, P <0.05); G in < / sub>-stage ovarian cancer tissue Ub protein expression was significantly the above G 1 , G 2 grade the significant difference (x 2 = 3.964, P <0.05); metastatic lymph nodes in Ub protein expression was significantly higher than those without lymph node metastasis organization, the difference was statistically significant (x 2 = 6.269, P <0.05) . 3 in epithelial ovarian cancer tissues of CyclinB1 expression with histological grade, clinical stage, lymph node metastasis-associated (P <0.05); histological type was no significant correlation (P> 0.05). III, Ⅳ of ovarian cancer tissue CyclinB1 protein expression was significantly higher than Ⅰ, Ⅱ stage, the difference was statistically significant (x 2 = 5.520, P <0.05); of G 3 < / sub>-stage ovarian cancer tissue CyclinB1 protein expression was significantly the above G 1 G 2 level, the difference was statistically significant (x 2 = 4.217, P <0.05); metastatic lymph nodes the CyclinB1 protein expression was significantly higher than those without lymph node metastasis tissue, and the difference was statistically significant (x 2 = 6.226, P <0.05) . 4 ovarian cancer tissue Ub and CyclinB1 protein expression: Ub of protein and CyclinB1 protein 7 cases were positive expression in 50 cases of ovarian cancer tissue, 19 cases were negative, Ub protein expression CyclinB1 protein negative expression by 10 cases, Ub protein negative expression CyclinB1 positive expression of 14 cases, the results indicate that the expression levels of both show a high degree of consistency by the Spearman rank correlation analysis, the correlation coefficient r = 0.301, P = 0.033, indicating that Ub and CyclinB1 protein expression in ovarian cancer tissue is positively correlated. Conclusion 1. Epithelial ovarian cancer tissue from normal ovarian tissue, benign epithelial ovarian tumor tissue to of Ub and CyclinB1 protein positive expression rate gradually increased, and more expressed in later clinical stage, histological grade poor lymph node metastasis in ovarian cancer, suggesting that both high expression may contribute to the occurrence of ovarian cancer development, invasion and metastasis. In ovarian cancer tissue increased with the CyclinB1 protein increased Ub protein expression, and a positive correlation between expression of of Ub and CyclinB1 in epithelial ovarian cancer tissue, suggesting that the two may be in the process of the development of ovarian cancer There is a synergistic effect. 3. Detection Ub and CyclinB1 of protein expression levels may determine the degree of malignancy of epithelial ovarian cancer is important.
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