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Mizolastine experimental mouse skin and spleen lymphocytes produce and release LTB4 and inhibition of IL-5

Author: ZhouZongLi
Tutor: FangHong
School: Zhejiang University
Course: Dermatology and Venereology
Keywords: Mizolastine Leukotriene B4 Interleukin- 5
CLC: R965
Type: Master's thesis
Year: 2004
Downloads: 55
Quote: 0
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Abstract

Mizolastine (mizolastine) is a novel imidazole derivatives of styrene, it is rapid onset, long duration of action, play efficient H1-receptor antagonism. Meanwhile, the drug has inhibiting 5 - lipoxygenase (5-lipoxygenase) activity, thereby inhibiting the metabolism of arachidonic acid-induced inflammatory effects. And IgE-mediated allergic diseases related pathological complex mechanisms in addition to mast cells to release histamine, but also the release of other inflammatory mediators, including cytokines, chemokines, neuropeptides, enzymes and so on. Therefore, the clinical H1-receptor antagonist alone for some allergic skin treatment is often ineffective. In recent years, 5 - lipoxygenase-mediated arachidonic acid metabolites leukotriene and TH2 type cytokines in these intractable role in inflammatory diseases received much attention. This experiment observation: ① the SP mizolastine mice induced LTB4 production and release of the local tissue, and inhibition of IL-5; ② mizolastine spleen of OVA-induced release of LTB4 and inhibition of IL-5, in order to understand the pathogenesis of inflammatory skin diseases, anti-inflammatory effect of revealing Mizolastine and clinical applications. : Have a: a master's thesis _ people hurried groups: Group 1: saline pretreatment intradermal injection of saline Group 2: saline pretreatment ten intradermal injection of SP group 3: 3.0m pair g microphone wow Sting intradermally administered pretreatment SP group 4:10. Om speaker microphone wow Sting g orally pretreated ten intradermal injections speaker SP group 5:10.0 m g loratadine orally given its pre-ten intradermal injection of SP Group 6: 3m Miao g dexamethasone administered intradermally SP1.2 pretreatment experiments mice were in each group with saline, different doses and a control drug Mi wow Sting gavage for 30 minutes in a small pre- rat back skin intradermal SP loolunol / only 5 minutes after the first batch of mice were sacrificed, the skin is made for injection site tissue homogenate levels of LTB4 determined EUSA competition; 24 hours, a second batch of mice were sacrificed, the same procedure before the competition ELISA, IL-5 levels. Determination of EUSA competition and in strict accordance with the measured value converter LTB4 and IL-5 product kit instructions 1.3 Statistical analysis of experimental data for x ± s, and SPSS10.0 statistical software, using t-test and ANOVA test . P lt; 0.05 was considered statistically significant difference. 2 Results 2.1 Sham real face skin homogenate levels of LTB4 (Table l) Group 1 LTB4 1.88p measured value of 5.53 years of age and disabilities, compared with group 2, there are two significant differences between the Iraqi lt; 0 .01), suggesting that SP intradermal injection of local skin tissue can be induced LTB4 production and release; Group 3 and Group 4 mouse skin tissue LTB4 levels were 3.11 ± 0.78 pg / ml, 1.23 ml ± 0.29p to stay , compared with group 2, there are significant differences in Iraq lt; 0.05), suggested microphone wow SP Sting significantly inhibited in mice induced by local production and release of LTB4 skin effect, the inhibitory effect is more obvious with the increase of the dose. Group 4 compared with group 5 there was a significant difference (P lt; 0 .01), prompted loratadine partial inhibition of SP-induced mouse skin tissue release LTB4 role than Mi wow Sting weak, group 4 and group 6 showed no significant difference (P gt; 0.05), prompted lomg suck g dose microphone wow Sting, the inhibition of local SP induced murine skin tissue LTB4 production and release of the role and 3m pair g dexamethasone considerably. No. 2 River lJ a Soviet Union, the two master thesis hasty Table 1: Skin tissue levels of LTB4 comparison group Group l Group 2 Group 3 Group 4 Group 5 Group 6 number of cases LTB4 (Dg/.1) 0 .23 ± 1.883 ± 0.135 .53 .23 .11 ± 0.781 ± 1.270 ± 0.293 .90 .76 disabilities 0.36206 plant 062,062.2 mouse skin tissue levels of IL-S Comparison (Table 2) Determination of group 1 IL-5 value of 179.6 Ban 46.25p Peng ml, compared with group 2, there are two significant differences between (P lt; 0.01), prompted SP intradermal injection in mice can induce local skin tissue IL-5 production and release; Groups 3 and Group 4 mouse skin tissue IL-5 levels were 84.78 ± 1 s.49pg/ml, 34.29 ± 1 1 .00pg/ml, compared with group 2, there is a significant difference (P lt; 0.05), Tip Mi Mile Sting significantly inhibited SP-induced mouse skin tissue locally produced and released the role of IL-5, and its inhibition increases with dose and more obvious. Group 4 compared with group 5 there was a significant difference (P lt; 0.01), prompted microphone wow Sting partial inhibition of SP-induced mouse skin tissue to produce and release the role of IL-5 was significantly stronger than loratadine, Group 4 compared with the group of six was no significant difference (BU 0.05), suggesting 10m speaker microphone wow Sting g dose, the inhibition of local SP-induced murine skin tissue to produce and release the role of IL-5 and 3m pair g dexamethasone considerably. Table 2: Skin tissue levels of IL-5 Comparison (two decimal places) group number of cases IL - 5 (Pg/.1) 17.49 ± 4.3166 group 5 group 6179.62 ± 46.2584.78 18.4934.29 ± 1 1.00127 ± .74 ± 43.2723.82 ± 11.11 No. 3 who, l f little less hasty second part of the master thesis microphone wow Sting on OVA-induced release of LTB4 and spleen inhibition of IL-5 1 Materials and methods 1.1 Animal groups Balb / c mice, 5 to 7 weeks old, weighing 19 a 219 clean grade, male and female, from Zhejiang University School of Medicine Experimental Animal Center. Mice were divided into eight groups: Group l: without any pretreatment group 2: solvent DMSO pretreatment group 3: 10.5 Miles wow solvent DMSO Sting 0.1 HS M pretreatment group 4: solvent DMSO microphone wow Sting 1.0 M Bu pretreatment group 5: Solvent DMSO microphone wow Sting 10.0 HS M pretreatment group 6: solvent DMSO ten chlorpheniramine 10.0 Lin M pretreatment group 7: solvent DMSO loratadine 10.0 Lin M pretreatment group 8: solvent DMSO Lin M dexamethasone pretreatment 1.2 1.0 Real Face processes were first and skillfully constructed day OVA sensitized mice injected mice, and then under sterile conditions spleen lymphocytes were separated and adjust cell concentration of 2x106 / m1, and group 3 to group 8 in the dish were added in different concentrations and a control drug Mi wow Sting pretreated with OVA stimulated again 30 minutes, 1 hour, collected by centrifugation and the supernatant was measured by ELISA competition levels of LTB4 After 36 hours, the same method IL

Mizolastine experimental mouse skin and spleen lymphocytes produce and release LTB4 and inhibition of IL-5

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