Dissertation > Excellent graduate degree dissertation topics show

Expression and Significance of NF-κB and VEGF in Renal Cell Carcinoma

Author: DiShuiLong
Tutor: SunHao
School: Jiangsu University
Course: Surgery
Keywords: renal cell carcinoma nuclear factor-KappaB vascular endothelial growth factor microvessel density immunohistochemistry
CLC: R737.11
Type: Master's thesis
Year: 2008
Downloads: 96
Quote: 0
Read: Download Dissertation


Objective:To investigate the expression of nuclear factor- kappaB (NF-κB)and vascular endothelial growth factor(VEGF)correlate with clinicopathological stage and angiogenesis in primary renal cell carcinoma(RCC).Methods:Immunohistochemistry was used to detect the expression of NF-κB p65 and VEGF in 40 cases of RCC and 10 cases of renal tissue. Microvessel density(MVD)was assessed by immunohistochemical staining for endothelial cells using anti-CD34 monoclonal antibody.RCC cases were divided into early staging group(Robson stagingⅠ、Ⅱ)and advanced group(Robson stagingⅢ、Ⅳ).Chisq test,student t test, Spearman rank correlation test were used for data statistic analysis.Result:(1)26 of 40 RCC were positive for NF-κB p65 protein with a 65.0%positive rate,but there was lower expression in normal renal tissue,Statistic significance existed between the two groups(P<0.01). The expression of NF-κB p65 were positive in 17 cases(85%)and negative in 3 cases(15%)of 20 cases of advanced RCC,were positive in 9 cases(45%)and negative in 11(55%)of 20 cases of early staging RCC, Statistic significance existed between the two groups(P<0.05).(2)The positive rate of VEGF in RCC(27/40;67.5%)was significantly higher than that in normal renal tissue(20.0%,P<0.05).The expression of VEGF were positive in 17 cases(85%)and negative in 3 cases(15%) of 20 cases of advanced RCC,were positive in 10 cases(50%)and negative in 10(50%)of 48 cases of early staging RCC,Statistic significance existed between the two groups(P<0.05).(3)MVD parameter was 63.87±19.51 in RCC and 43.58±3.11 in control group, Statistic significance existed between the two groups(P<0.05).MVD parameter was 78.16±16.84 in advanced group and 58.16±14.73 in early staging group,Statistic significance existed between the two groups (P<0.05).NF-κB negative staining tumor(55.44±4.72)showed a significant lower mean MVD per microscopic field than NF-κB positive tumor(77.08±15.59),Statistic significance existed between the two groups(P<0.01).The same result was found on VEGF(P<0.01).MVD parameter was 86.54±10.76 in both NF-κB and VEGF positive staining group,was 67.04±2.42 in either NF-κB or VEGF positive staining group, was 51.64±3.45 in neither NF-κB nor VEGF positive staining group. Statistic significance existed among the three groups(P<0.01).(4)22 of 40 RCC were both NF-κB p65 and VEGF positive,9 of 40 RCC were both NF-κB p65 and VEGF negative.The expression of NF-κB p65 has a positive correlation with that of VEGF in RCC(P<0.01,r=0.498).(5) NF-κB and VEGF expression is highly related with the prognosis of RCC patients.Conclusions:NF-κB and VEGF was activated abnormally in RCC, especially in advanced RCC.The expression of NF-κB and VEGF correlated with angiogenesis in RCC.NF-κB and VEGF may alter the biological behavior of RCC by prompting angiogenesis.NF-κB may positively adjust high VEGF expression in RCC.The expression of NF-κB and VEGF may reflect the differentiation level of RCC and is highly related with the prognosis of RCC patients.They may be a new target for curing of RCC and serve as key for researching mechanism of RCC.

Related Dissertations

  1. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  2. The Study of Kisspeptin/GPR54 System on the Reproductive Regulation in the Hen’s Hypothalamus-Pituitary-Ovary Axis,S831
  3. Study on the Digestive Tube During the Embryonic Stage of Gushi Chicken,S831
  4. A Comparative Study of prawns, crabs, Spiroplasma disease immunohistochemistry and pathology,S945
  5. The Expression of MMP-2、 VEGF and Ki-67 and Human Glioma Grade,R739.41
  6. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  7. Expressions of PRL-3 and p27 in Gastric Carcinoma and Their Clinical Significance,R735.2
  8. Expression of hnRNP A2/B1 and p21WAF1 Proteins in Lung Cancer and Their Clinical Significance,R734.2
  9. The Effects of RIP on the Immune Function of Renewal Rats Without Colostrums,S853.7
  10. Study on the Expression and Correlation of Metallothionein and Matrix Metalloproteinase-2 in Non-small Cell Lung Cancer,R734.2
  11. The Research on Treatment of Lentivirus-mediated hVEGF-165 Gene Transferring Mesenchymal Stem Cells on Rat Hind Limb Ischemia,R329
  12. Expression and Their Significance of Apoptosis Regulatory Factors Survivin、XIAP and c-Jun、NF-κB in Epithelial Neoplasm of Ovary,R737.31
  13. Expression and Clinical Significance of Survivin and YAP in Cervical Carcinoma,R737.33
  14. Expression and Clinical Significance of PTEN and Caspase-7 in Gastric Cancer and Precancerous Lesion,R735.2
  15. Cloning and Expression Analysis of NCCRP-1 AND IL-10 Genes in Grass Carp (Ctenopharyngodon Idellus),S917.4
  16. Expression and Antibody Preparation of sglt1 from Cyprinus CarpioL.,S917.4
  17. Angiogenic Effect of Platelet-rich Plasma at Early Stage of Adipose Grafts,R329
  18. The Effect of Hepatic Denervation on Liver Regeneration after Partially Hepatectomy in Rats,R363
  19. BMP2 and VEGF165 Genes Transfected Rat Bone Marrow Mesechymal Stem Cells Simultaneity Expression,R329
  20. Expression and Correlation of Caspase-3 and Cx43 in Myocardial Infarction with Different Narrow Degree Coronary Artery,R542.22
  21. The Expression and Relationships of Bmi-1 and c-myc in Diffuse Large B-cell Lymphoma Tissues,R733.1

CLC: > Medicine, health > Oncology > Genitourinary tumors > Urinary tumors > Kidney,renal pelvis tumor
© 2012 www.DissertationTopic.Net  Mobile