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Background and purpose: salivary gland tumors are the most common tumors of the head and neck, and its incidence is on the rise, especially malignant salivary gland directly endanger the lives of patients and affect the patient's quality of life. Currently, the diagnosis of salivary gland tumors, has made great strides in the treatment, but the diagnosis and differential diagnosis of certain salivary gland tumors are still plagued by the majority of pathologists, also an urgent need to determine the ideal tumor prognosis and guide treatment tumor clinical marker. Pleomorphic adenoma (pleomorphic adenoma, PA) is the most common salivary gland tumor, accounting for 60% of all salivary gland tumors. PA pathological features: enveloped by epithelial, myoepithelial and mesenchymal components. Part of the PA can be seen by the epithelial and myoepithelial sieve-like structure, easy to be confused with adenoid cystic carcinoma, leading to difficulties in the differential diagnosis, which is the majority of the pathologist long unresolved problem. Common malignant epithelial salivary gland tumors, including mucoepidermoid carcinoma (MEC) and adenoid cystic carcinoma (ACC) and acinar cell carcinoma (ACCa). Tenascin-C (TN-C) is an extracellular matrix polymer glycoprotein, with the formation of the embryo, the occurrence and development of tumor tissue wound repair and fibrosis, cellular aging process has a close relationship. The study showed that the TN-C have different levels of expression in a variety of tumors and in normal tissue and benign tumors do not express or rare expression. Salivary gland tumors, Westernoff observed using immunohistochemical methods TN-C expression in salivary gland tumors, suggesting that there is a certain value of TN-C in the differential diagnosis of pleomorphic adenoma and adenoid cystic carcinoma. CD9 is a type of white blood cell surface-associated protein secretion, and cell proliferation, migration, and metastasis of the tumor cells are closely related. The study showed that CD9 expression in benign tumors and normal tissues express relatively higher expression in malignant tumors is relatively low. For oral tumors, Kikuo Sakmaoto immunohistochemical method to detect the expression of CD9 in parotid gland tumors, the results show that the the CD9 available as identify indicators of benign and malignant parotid gland tumors. The purpose of this study is to explore the relationship of the TN-C and CD9 salivary gland tumor, at the same time to explore both the value of the differential diagnosis of salivary gland tumors, looking for to provide experimental evidence for the occurrence of salivary gland tumors, differential diagnosis and therapeutic target. Method: 1. Using immunohistochemistry SP method detected 25 cases of pleomorphic adenoma, 25 patients with adenoid cystic carcinoma, 20 cases of mucoepidermoid carcinoma, 10 cases of acinar cell carcinoma and 10 cases of normal salivary gland tissue TN-C and protein CD9. By in situ hybridization to detect 25 cases of pleomorphic adenoma, 25 cases of adenoid cystic carcinoma, 20 cases of mucoepidermoid carcinoma, 10 cases of acinar cell carcinoma and 10 cases of normal salivary gland tissue in the TN-C and CD9mRNA expressed. 3. Statistical analysis: the application SPSS 17.0 statistical software processing, the rate was used to compare the χ2 test (chi-square), correlation analysis using Spearmen correlation analysis. The inspection level α = 0.05. Results: 1.TN-C protein negative expression in 10 cases of normal salivary gland tissue. Pleomorphic adenoma, TN-C protein expression is mainly found in the glandular epithelium, cytoplasmic brownish yellow, the positive rate was 16% (4/25); adenoid cystic carcinoma, TN-C protein in cancer tissue showed diffuse expression in part was spotty expression, the positive rate was 64% (16/25); the mucoepidermoid carcinoma epidermoid cells and mucus cytoplasm both positive expression, the positive rate was 40% (8 / 20); acinar cell carcinoma, TN-C protein found in the cytoplasm of cancer cells was 50% (5/10). TN-C protein positive expression rate in adenoid cystic carcinoma was significantly higher than pleomorphic adenoma, a statistically significant difference (χ2 = 12.000, P = 0.001 lt; 0.05), while in adenoid cystic carcinoma with mucus epidermoid carcinoma, no statistically significant difference in the expression in the acinar cell carcinoma (χ2 were 2.571 and 0.583, P = 0.109 and 0.445 gt; 0.05). In normal salivary gland tissue, CD9 protein expression were mainly seen in the ductal epithelium and a handful of glandular epithelium, cytoplasmic brownish yellow, the positive rate was 100% (10/10); pleomorphic adenoma, CD9 protein found in glandular epithelium and squamous epithelial cytoplasm and envelope expression range limitations, myoepithelial no expression, the positive rate was 32% (8/25); adenoid cystic carcinoma, CD9 protein in cancer tissue showed diffuse expression, the part was spotty expression, the positive rate was 80% (20/25); the mucoepidermoid epidermoid carcinoma cells and mucus cytoplasmic expression were positive, the positive rate was 70% (14/20); in acinar cell carcinoma of the the CD9 protein found in the cytoplasm of cancer cells, the positive rate was 90% (9/10). CD9 protein in adenoid cystic carcinoma, the positive expression rate was significantly higher than the pleomorphic adenoma, and the difference was statistically significant (χ 2 = 11.688, P = 0.001 lt; 0.05), adenoid cystic carcinoma and mucoepidermoid carcinoma acinar cell carcinoma, the expression differences statistically significant (χ2 were 0.602 and 0.503, P = 0.438 and 0.478 gt; 0.05). 3. TN-CmRNA negative expression in 10 cases of normal salivary gland tissue; pleomorphic adenoma, TN-CmRNA mainly seen in the tumor cells, the cytoplasm is deep blue, the positive rate was 12% (3/25); in adenoid cystic carcinoma, TN-CmRNA cancer tissues showed diffuse expression, the positive rate was 56% (14/25); mucoepidermoid carcinoma epidermoid cells and mucus cells cytoplasm were positive expression positive rate in acinar cell carcinoma the CD9 protein found in the cytoplasm of cancer cells, and the positive rate was 40% (4/10), 35% (7/20); Significantly higher than in the positive expression rate of of TN-CmRNA adenoid cystic carcinoma in pleomorphic adenoma, a statistically significant difference (χ 2 = 10.784, P = -0.001 lt; 0.05) in adenoid cystic carcinoma with mucus. epidermoid carcinoma, no statistically significant difference in the expression in the acinar cell carcinoma (χ2 were 1.969 and 0.732, P = 0.161 and 0.392 are gt; 0.05). In normal salivary gland tissue, CD9mRNA positive expression mainly seen in the ductal epithelium, the cytoplasm is deep blue, the positive rate was 100% (10/10); pleomorphic adenoma, CD9mRNA mainly found in tumor cells, the cytoplasm is dark blue, the positive rate was 20% (5/25); adenoid cystic carcinoma, CD9mRNA mucoepidermoid epidermoid carcinoma cells in cancer tissue showed diffuse expression, the positive rate was 72% (18/25); were positive and mucus cytoplasmic expression, the positive rate was 70% (14/20); CD9mRNA acinar cell carcinoma found in the cytoplasm of cancer cells, the positive rate was 80% (8/10). Significantly higher than in the positive expression rate of CD9mRNA adenoid cystic carcinoma in pleomorphic adenoma, a statistically significant difference (χ 2 = 13.607, P lt; 0.001), adenoid cystic carcinoma and mucoepidermoid carcinoma, adenosquamous the bubble cell carcinoma in the expression of the difference was not statistically significant (χ2 0.022 and 0.239, respectively, P = 0.883 and 0.625 are gt; 0.05). 5.80 cases of TN-C protein expression with TN-CmRNA salivary gland tumors, while the positive rate was 37.5% (30/80), at the same time the negative rate of 40% (32/80), expression concordance rate of 88.8%. The correlation analysis between the two has a very significant positive correlation (r = 0.734 P = 0.03); 80 cases of salivary gland tumors the CD9 protein with CD9mRNA of expression, while the positive rate was 68.7% (55/80), while a negative rate 12.5% ??(10/80), expression consistent rate of 88.8%. Correlation analysis between the two have very significantly positively related (r = 0.676P = -0.03 The). Conclusion: 1.TN-C negative expression in normal salivary gland tissue have different levels of expression in salivary gland tumors, suggesting that TN-C may have some correlation with salivary gland tumors occur; TN-C on The differential diagnosis of salivary gland tumors among certain sense. 2.CD9 positive expression in normal salivary gland tissue was 100% higher than the expression in the salivary gland tumors, suggesting that CD9 may have some correlation with salivary gland tumors occur; CD9 differential between salivary gland tumors diagnosed with a certain sense. Four kinds of salivary gland tumors between TN-C protein, CD9 protein TN-C mRNA CD9mRNA significant correlation; prompted TN-C, CD9 gene and protein levels of salivary gland tumor occurrence and development play a regulatory role .
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