Dissertation > Excellent graduate degree dissertation topics show

Tanshinone ⅡA Attenuates Seawater Aspiration-induced Lung Injury by Inhibiting Macrophage Migration Inhibitory Factor

Author: ZhangYong
Tutor: JinFaGuang；ZhangBo
School: Fourth Military Medical University
Course: Internal Medicine
Keywords: Seawater Lung injury Tanshinone Macrophage migration inhibitory factor phospho-NF-κB p65 IL-6 TNF-α
CLC: R285
Type: Master's thesis
Year: 2011
Downloads: 21
Quote: 0
Read: Download Dissertation

Abstract


Background: Salvia Lamiaceae plants, its roots can be made after artificial drying traditional Chinese herbal medicine, is widely used in the clinical treatment of various cardiovascular and cerebrovascular diseases. In Salvia test a variety of water-soluble or fat-soluble compounds, these active ingredients not only with the expansion of the coronary arteries, improving myocardial blood supply, blood improve microcirculation, as well as anti-oxidation, anti-inflammatory, anti-apoptotic variety of pharmacological effects . Tanshinone (tanshinone Tan) is a class of fat-soluble ingredients, Salvia miltiorrhiza the (saliva mitiorrhizabge) rhizome extract containing ortho-quinone or quinone structure, the general term for tanshinone compounds. Especially water-soluble derivative of tanshinone formed by sulfonation - tanshinone Ⅱ A sulfonate (TIIA) showed more kinds of biological activity, have been widely used in the clinical treatment of cardiovascular and cerebrovascular diseases, a large number of experiments confirmed TIIA also has a strong anti-tumor, anti-inflammatory and antioxidant pharmacological effects. There are research reports, a protective effect of T Ⅱ A on LPS-induced animal's lung injury, its mechanisms, including inhibition of NF-KB activation, reducing the activity of phospholipase A2 (PLA2)'s, to reduce nitric oxide and other inflammation factors and mitigate lung injury of tissue inflammation; cells against oxidation and cell apoptosis alleviate lung tissue damage and improve microvascular permeability, Tan Ⅱ A, however, has not been reported flooded the role of female lung injury and mechanisms. Sea water drowning pulmonary edema (PE2SWD) showed hypoxemia and pulmonary edema, the mechanism of injury, including severe hypoxia causes abnormal lung function, sea water surfactant destruction of alveolar epithelial cells, seawater led to the induction of inflammatory mediators neutrophils gathered dissolving enzymes and oxygen free radicals excessive release of seawater cause direct injury of alveolar epithelial cells and lung microvascular endothelial cells, changes in water channels and ion channels, endothelin, calcitonin and heme oxygenase changes. Still lack clinical the ideal treatment measures. The previous study found that dexamethasone can inhibit inflammation play the prevention PE2SWD role Tan Ⅱ A can also protect LPS-induced lung injury by suppressing inflammation, however TIIA flooded female lung injury effect has not yet been reported. Therefore, this experiment in general and cell experiments with cell-mediated immunity, and molecular biology technology to reveal the mechanism of drowning inflammation of the lung injury, to investigate the protective effect and mechanism of TIIA flooded female lung injury. Objective: Rat sea flooded the drowning-induced lung injury model; 2. Explore the the inflammation mechanism for sea water drowning lung injury; 3. Seawater drowning-induced lung injury to reveal TIIA protection mechanisms. SWD ALI animal model methods: 1. Experimental inhaled through the lungs of rats to endotracheal intubation appropriate amount of seawater, copied successfully meet the actual situation seawater drowning and consistent with ALI diagnostic criteria .2 24 rats were divided normal control group, the seawater drowning group, the the tanshinone intervention group and the the tanshinone control group. Drowning group intratracheal injection of 4ml/kg seawater, tanshinone group rats within 30 minutes after intraperitoneal modeling to inject TIIA (25 mg / kg), PaO2 changes detected at different time points, HE staining by detecting changes observed destruction of lung tissue structure, detection of lung tissue wet to dry ratio changes observed lung tissue edema, through the detection of MPO activity validates the lung tissue inflammation cell infiltration, and Western-blot analysis and ELISA method respectively detected MIF in lung tissue and plasma expression . Experiment (a) NR8383 cells into normal control group, seawater drowning group the seawater ISO-1 control group, the seawater TIIA intervention group and TIIA control group. Seawater drowning group cell supernatants by adding 25% water, the seawater TIIA intervention group cell supernatant was added to 10 ug / ml TIIA, seawater ISO-1 cell supernatant was added to ISO-1 (50uM). Take 4-hour time point is detected by ELISA of MIF in cell supernatants, the expression of IL-6 and TNF-α in. Western-blot analysis to detect the expression of phospho-NF-κB p65. The NR8383 cell of the experiment (b) divided into normal control group, rMIF intervention the group, TIIA intervention group. rMIF (25ug/ml), TIIA rMIF cell supernatant of the intervention group was added was added to the intervention group was added to the cell supernatant after MIF (25ug/ml) TIIA (10ug/ml), take the cell supernatant of the 4-hour time point is detected by ELISA in the MIF, IL-6 and TNF-α expression. Western-blot analysis to detect the expression of phospho-NF-κB p65. Results: 1. Seawater flooded the female lung injury model successfully reproduced. 2 relative to the normal control group, seawater drowning rats, pulmonary edema, hypoxemia, and accompanied by the aggregation of lung tissue damage and inflammatory cells, while the MIF high expression in the lung tissue, MPO activity along with it. Tanshinone lung injury after the intervention reduced MIF expression is suppressed, and is accompanied by reduced MPO activity. Experiment (a) sea water drowning group cell MIF secretion increased, a corresponding increase in phospho-NF-κB p65 and IL-6 and TNF-α expression. ISO-1 intervention group MIF expression in normal activity is inhibited, of phospho-of NF-κB p65 in IL-6 and TNF-α expression relative to sea drowning group significantly reduced cell MIF in the seawater TIIA intervention group reduced secretion of phospho -NF-κB p65 and IL-6 and TNF-α expression is reduced. Cytokine expression TIIA control group group relative to the normal control group, no significant change. Experiment (b) rMIF intervention group cell supernatants phospho-NF-κB p65 and IL-6 and TNF-α expression increase, rMIF TIIA intervention group cell supernatant phospho-NF-κB p65 and IL-6 and TNF- α relative to the decrease in the expression of rMIF intervention group. Conclusion: the MIF-induced inflammation mechanisms involved in the occurrence of seawater drowning-induced lung injury, the tanshinone possible drowning lung injury by inhibition of MIF protected flooded.

Related Dissertations

  1. Inhibition of RAW264.7 Macrophage Inflammatory Cytokines Release by Guggulsterone,R285
  2. Infectious Bursal Disease Virus Particle Vaccine Research and Its Immune Efficacy Evaluation,S858.31
  3. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  4. Preparation of Monoclonal Antibody to Porcine TNF-α and Expression of TNF-α in PAM Infected with PRRSV,S858.28
  5. Live alone the study of parasitic soup rabbit lumbar disc herniation model in vivo tumor necrosis factor in rats,R285.5
  6. The Expression and Significance of Nuclear Factor Kappa B (NF-κb) and IL-6 in the Degenerated Cervical Intervertebral Disc,R681.55
  7. Cysteamine on T Lymphocyte Proliferation in Post-Operation Goats and Its Mechanism Underlying,S858.27
  8. The Sequence Analysis of RNA Components from Lactobacillus Metabolites and Parts of the Biological Function,R378
  9. Cloning and Expression Analysis of NCCRP-1 AND IL-10 Genes in Grass Carp (Ctenopharyngodon Idellus),S917.4
  10. Exploreing the Mechanism of Xieshifeiaifang Ii on Treating Lung Cancer with Pleura Effusion,R734.2
  11. Expression of Protease-activated Receptor-2 in Lung Injury of Rats with Different Ventilatory Volumes,R563.8
  12. The Expression and Clinical Significance of TH17 Cells Related Cytokines in the Patients with Rheumatoid Arthritis,R593.22
  13. Diagnosis Value of Cytokine Levels and TB-Ab in Serum in Children Tuberculosis and Latent Tuberculosis Infection,R725.1
  14. Glucocorticoid treatment of bronchial asthma patients serum IL-18, IL-33 levels in,R562.25
  15. The Expression and Inflammatory Correlation of md2 in the Brain of Rats after Inducing Severe Acute Pancreatitis,R576
  16. The Expression of Angiopoietin-2 in Diabetic Nephropathy and Correlated with Proteinuria,R587.2
  17. Function Research of Th1/2、Th17 Cells and CD4~+ IL-10~+ Treg Cells of Peripheral Blood in AR and DE,R765.21
  18. Ti / RuO 2 -IrO 2 -SnO 2 -Sb 2 O 5 Preparation and Application of anode,X703
  19. Anti- macrophage migration inhibitory factor monoclonal antibody identification and protective mechanisms Sepsis Study,R459.7
  20. Effects of Tanreqing Injection on Acute Lung Injury Induced by Endotoxin in Rats,R563
  21. The Expression of IL-1β、CD34 and Their Significance in the Intervertebral Disc Degeneration of Subcutaneous Nicotine Injection of Rats,R681.53

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology
© 2012 www.DissertationTopic.Net  Mobile