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Influence of Silencing of Homeobox Gene NKX3.1 by RNA Interference on LNCaP Prostate Cancercell Lines

Author: ChenGang
Tutor: ZhangYuanFang；ZuoLianXi；ZhengPing；WangXiang
School: Fudan University
Course: Surgery
Keywords: Prostate Cancer Cancer Androgen Testosterone Finasteride Cape acid sequence analysis of oligonucleotide Plasmid RNA interference Small interfering RNA NKX3.1 LNCaP Nude
CLC: R737.25
Type: PhD thesis
Year: 2005
Downloads: 165
Quote: 0
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Abstract


Background Prostate cancer is the male reproductive system of Western countries, the most common malignancy. In the United States, prostate cancer accounts for male cancer incidence and mortality in the first second only to lung cancer. As the number of elderly population in our growth and raising the level of diagnosis, prostate cancer accounted for the proportion of patients increased year by year. Subclinical prostate cancer early, more than 40% of the patients when first diagnosed metastasis has occurred, late in the disease, cancer cells lose androgen dependent, androgen-dependent prostate cancer will become androgen-independent prostate cancer, a shorter survival time of these patients, a serious threat to the health of middle-aged men, life and quality of life. Therefore, prevention and treatment of prostate cancer is placed in front of health workers needed to solve the problem. Since 1941, Huggins made androgen dependent prostate cancer theory has several decades, and androgen deprivation therapy in prostate cancer has been a very positive effect, and with the deepening of the study, the emergence of a number of anti- androgen drugs, among them type 2 5α reductase inhibitor finasteride (common name: Proscar). Finasteride is currently widely used in benign prostatic hyperplasia (BPH) treatment, inhibition of the conversion of testosterone to dihydrotestosterone, and thus inhibit prostatic hyperplasia, the glands shrink and eliminate prostate hyperplasia caused by lower urinary tract obstruction purposes. 2003 a period of 7 years has 18,882 people participated in the clinical trials showed that finasteride can inhibit or delay the occurrence of prostate cancer, showing finasteride for prostate cancer prevention potential. There vitro experiments showed that finasteride can also inhibit the growth of prostate cancer cells, including androgen-sensitive cell lines PC3 and DU145 and androgen-sensitive cell line LNCaP. To explore finasteride prostate cancer mechanisms, we used microarray studies finasteride prostate cancer LNCaP cells under the action of changes in gene expression, screening out some finasteride germane tumor suppressor role genes, including homeobox gene NKX3.1, such as tumor suppressor gene PTEN. Homeobox gene NKX3.1 in prostate cancer development has an important role, NKX3.1 loss and prostate cancer precancerous lesions - prostatic intraepithelial neoplasia (PIN) is closely related to, but clinical data show NKX3. a prognosis of prostate cancer, there is hope for prostate cancer prognosis. NKX3.1 in order to further clarify the important role of prostate cancer, we used RNA interference technology enables NKX3.1 expression of androgen-sensitive prostate cancer cell line LNCaP silence the expression of NKX3.1, NKX3.1 expression silencing on LNCaP research cell biology effects. Subsequently established NKX3.1 loss of expression in prostate cancer LNCaP cell line LNCaP (-NKX3.1), and make LNCaP cells in nude mice in vivo studies, observational NKX3.1 expression silencing on LNCaP cells tumorigenic effects of NKX3.1 as a promising prostate cancer tumor markers and targets for gene therapy provide a theoretical basis. The first part Microarray finasteride prostate cancer LNCaP cells under the action of changes in gene expression purposes

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Male genitalia tumors > Prostate cancer
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