Dissertation > Excellent graduate degree dissertation topics show

Adenovirus -mediated mouse liver sphingomyelin synthase SMS overexpression and atherosclerosis study of the relationship

Author: DongJiBin
Tutor: WuManPing
School: Fudan University
Course: Biochemistry and Molecular Biology
Keywords: Sphingomyelin (SM) Sphingomyelin synthase (SMS) Sphingomyelin hydrolase ( SMase ) Atherosclerosis (AS) High-density lipoprotein (HDL) Apolipoprotein (Apo) Atheroma plaque Aortic adenovirus vector RT-PCR Thin layer chromatography (TLC) FPLC western-blotting SR-BI Gel scan image analysis
CLC: R543
Type: PhD thesis
Year: 2007
Downloads: 142
Quote: 0
Read: Download Dissertation

Abstract


The study was focused on the following two specific aims:1.To establish a mice model that over expression SMS using adenovirus vectors,2.Characterize the influence of SMS over-expression on atherogenesis.Adenovirus vectors carrying Sphingomyelin Synthase(SMS1 or SMS2)were constructed,amplified and purified.Intravenous injection of adenovirus into 8-week C57BL/6J mice generated an SMS over-expression model.Establish of such model was confirmed by both mRNA level examination(RT-PCR)and protein level examination(SMS activity assay).Based on the SMS over-expression model,following alterations were checked in order to establish the relationship between SMS Overexpression and atherogenesis:1.Level of plasma lipid(HDL-Cholesterol、phosphatidylcholine、SM、TG,total cholesterol)2.Level of lipid in liver(cholesterol,TG,SM)3.Plasma HDL and non-HDL concentration;4.SR-BI and ABCA1 level in liver5.HDL turnover test and AS lesionThe over-expression of SMS resulted into following changes:1.hepatic SMS1 and SMS2 mRNA levels were significantly increased,2.5- and 2.7-fold respectively;p<0.0012.hepatic SMS1 and SMS2 activity were significantly increased,2.1- and 2.3-fold respectively;p<0.001.3.Decrease the SM content and the Cholesterol content in HDL.And also increase the SM content and the Cholesterol content in non-HDL particles. HDL-SM was decreased 42%or 38%separately(p<0.05),HDL-ch was decreased 27%or 25%separately(p<0.05);non-HDL-SM increased 50%or 35%separately(p<0.05),non-HDL-ch was increased 64%or 61%separately (p<0.05).4.Apo B levels was significantly increased(p<0.01),while Apo A-I levels no change.5.Liver HDL receptor SR-BI expression level was increased 50%or 55% separately,and then enhanced the ability to uptake HDL-cholesterol ester selectively.There was no change to ABCA1 expression level.6.The SMase-induced aggregation of non-HDL lipoprotein was increased (p<0.0001).7.Plasma HDL clearance rates was increased(p<0.02).8.There was no functional difference between SMS1 and SMS2.To Conclude:1.Adenovirus-mediated SMS1 and SMS2 over-expression in mice increased lipoproteins atherogenic potential2.There were no significant functional difference between SMS1 and SMS23.SMS maybe a candidate target for the AS research or drug screening

Related Dissertations

  1. Serum A-FABP Levels and Its Expression in Atherosclerotic Plaques of Diabetes Mellitus,R587.1
  2. GIH cDNA Cloning and Mrna Expression in Litopenaeus Vannamei,S917.4
  3. Gene Prediction and Sequence Analysis of Insect OBP CSP and sid-1,Q78
  4. Isolation, Identification and Molecular Characteristics of Japane Encephalitis Virus in Henan Province,S852.65
  5. Studies on the Difference of PRRSV Infectious and PRRSV ADE,S858.28
  6. cDNA-cloning and Expression analysis of Phosphate Stress Induced Purple Acid Phosphatase Genes and transcription Factor Genes from Seeding Wheat,S512.1
  7. Study on Key Technology and Its Physiological Basis of Virus Elimination and Rapid Propagation of Phalaenopsis,S682.31
  8. Molecular Mapping of Downy Mildew Resistant Gene and Expression of Rs-AFPs in Radish (Raphanus sativus L.),S631.1
  9. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  10. Proliferation and Differentiation Effects of Different Culture Methods and Cell Factors on Mouse Spermatogenic Cells in Vitro,R329
  11. The Changes of Intimal Macrophages, Smooth Muscle Cells, Collagen, and Cpt-Ⅰ, GLUT-4 of Vascular Media in Human Lower Limb Artery with Atherosclerotic Stenosis,R543.5
  12. Effect of High Glucose on Chemokine Ligand 16 (CXCL16) Expression and Secretion in THP-1 Macrophages and Intervention of Aspirin in the Activity,R587.2
  13. The Detection and Significance of CK 19 mRNA and MUC1 mRNA in Peripheral Blood in Cervical Cancer Patients.,R737.33
  14. Correlation Research of Plasma Apolipoprotein CⅢ and High Sensitive C-reactive Protein with Atherothrombotic Cerebral Infarction,R743.33
  15. Role of G31P in Smooth Muscle Migration of A7r5 Cells Induced by Interleukin-8,R363
  16. Research of Biochemical Characteristics of Lipid Raft in Larval Midgut of Helicoverpa Armigera H(?)bner and the Immunodetection of Cadherin,S435.622.3
  17. Effect of HCO3-, K+ and HSO3- on rbcL and rbcS Gene Expression and Photosynthesis of Soybean Seedlings,S565.1
  18. The Impact of Histone Deacetylase Inhibitor Trichostatin a on Proliferation and Apoptosis of Porcine Granulosa Cells,S828
  19. The Effect of P-selectin on the Maturation of Dendritic Cells from Human Peripheral Blood,R543.5
  20. Primary Study on CLC1 Gene Identification and Its Function in Glycine Max, Salt-born Glycine Soja and Their Hybrid,S565.1
  21. Cloning of Celluase Gene cel6B from Lentinula Endodes and Its Expression in Escherichia Coil,S646.12

CLC: > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Vascular disease
© 2012 www.DissertationTopic.Net  Mobile