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Objective:To study the ethnic group distribution of HLA-DRB1alleles and elucidate the effect of gene polymorphism of HLA-DRB1alleles on familial aggregation of hepatocellular carcinoma(HCC) in high hepatocellular carcinoma incidence area of Guangxi, and to provide clues to seeking for the susceptible or antagonistic genes for hepatocellular carcinoma.Methods:153family members from33families which have had two or more than two patients with hepatocellular carcinoma(FHHC group),153family members from37families which have had only one patient with hepatocellular carcinoma(FOHC group) and153family members from59fimilies which have had none carcinoma patients(FNC group) were selected and matched as study object in the high hepatocellular carcinoma incidence areas of Guangxi. Polymerase chain reaction using sequence specific primer (PCR-SSP) was employed to tested HLA-DRB1*07, HLA-DRB1*09, HLA-DRB1*11, HLA-DRB1*12, HLA-DRB1*13, HLA-DRB1*14,and HLA-DRB1*15alleles; the ethnic distribution of HLA-DRB1alleles in the high hepatocellular carcinoma incidence areas of Guangxi and the correlation bewteen gene po1ymorphism of these alleles and familial aggregation of HCC was analysed by Statistical methods.Results:(1)The ethnic group distribution of HLA-DRB1*07,09,11,12,13,14,and15alleles in Han,Zhuang and Yao three main ethnic groups of Guangxi was that:HLA-DRB1*07was1.8%,0.9%and3.5%respectively(χ2=3.156,P=0.206)；HLA-DRB1*09was18.9%,22.1%and14.9%(χ2=2.578,P=0.276)；HLA-DRB1*11was9.0%,10.3%and7.0%(χ2=0.971,P=0.615)；HLA-DRB1*12was11.7%,17.5%and25.4%(χ2=7.256,P=0.027)；HLA-DRB1*13was0.9%,5.3%and5.1%(χ2=3.873,P=0.144)；HLA-DRB1*14was42.3%,21.9%and25.6%(χ2=13.702,P=0.001)；HLA-DRB1*15was35.1%,41.5%and43.0%(χ2=1.701,P=0.427).The result showed that there was significant difference in the genetic distribution of HLA-DRB1*12and HLA-DRB1*14among Han,Zhuang and Yao three main ethnic groups of Guangxi(P<0.05)(2)The gene frequency of allele HLA-DRB1*07in the FHHC group,FOHC group and FNC group was0.7%,2.0%and2.6%(P>0.05)respectively；HLA-DRB1*09was19.0%,23.5%and16.3%(P>0.05)；HLA-DRB1*13was2.6%,6.5%and3.8%(P>0.05)； HLA-DRB1*14was35.3%,28.1%and22.9%(P>0.05)；HLA-DRB1*15was43.1%,42.5%and35.3%(P>0.05)；HLA-DRB1*11was2.6%, 13.7%and11.1%(P=0.001,P<0.01)；HLA-DRB1*12was15.7%,13.7%and24.8%(P=0.036,P<0.05).The results showed that there was significant difference in the the gene frepuency of HLA-DRB1*11and HLA-DRB1*12among FHHC group,FOHC group and FNHC group.(3).The gene frequency of HLA-DRB1*07,09,11,12,13,14,and15these7alleles in HBsAg postive group and HBsAg negative group was respectively:HLA-DRB1*07was0%and2.6%(χ2=1.701,P=0.427)； HLA-DRB1*09was19.0%and19.9%(χ2=0.043,P=0.836)； HLA-DRB1*11was9.5%and9.0%(χ2=0.036,P=0.849)；HLA-DRB1*12was17.9％and18.4%(χ2=0.012,P=0.913)；HLA-DRB1*13was2.7%and4.8%(χ2=1.096,P=0.295)；HLA-DRB1*14was25.9%and30.1%(χ2=0.893,P=0.345)；HLA-DRB1*15was39.4%and42.2%(χ2=0.315, P=0.575).It showed that there was no significant difference in the the gene frepuency of HLA-DRB1*07,09,11,12,13,14,and15these seven alleles bewteen HBsAg postive group and HBsAg negative group.Conclusions:(1)HLA-DRB1*15and HLA-DRB1*14is high-frequency gene in population of Han,Zhuang and Yao three main ethnic groups in high HCC incidence areas of Guangxi,while HLA-DRB1*13and HLA-DRB1*07were low-frequence gene；The genetic distribution of HLA-DRB1*12and HLA-DRB1*14in Han,Zhuang and Yao three main ethnic groups of Guangxi has specifical ethnic characteristics.(2) HLA-DRB1*11and HLA-DRB1*12seems to be the antagonist genes of hepatocellular carcinoma in high HCC incidence areas of Guangxi, and their gene deletion may be the cause of leading to the familial aggregation of hepatocellular carcinoma in high HCC incidence areas of Guangxi.(3) There may be not significant correlation bewteen HLA-DRB1*07,09,11,12,13,14,15alleles and the susceptibility of HBV infection in high HCC incidence area of Guangxi.(4) Further analysis suggested that HLA-DRB1*11and HLA-DRB1*12may not use the way of reducing the susceptibility of HBV infection,but other immunce mechanism to reduce the susceptibility of HCC in high HCC incidence areas of Guangxi.
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