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Experimental Study of the Expression of Intestinal Dendritic Cells and Related Cytokines of Rat Model with Postinfectious Bowel Dysfunction

Author: ZhouZheng
Tutor: WangQiaoMin
School: Anhui Medical University,
Course: Internal Medicine
Keywords: Irritable Bowel Syndrome Dendritic cells Shigella Interleukin -1β Interleukin-4
CLC: R574
Type: Master's thesis
Year: 2010
Downloads: 85
Quote: 0
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Abstract


Background Irritable bowel syndrome (irritable bowel syndrome, IBS) is a chronic bowel syndrome, abdominal pain or discomfort associated with change in bowel habits, and no changes in intestinal histology and biochemical abnormalities, belonging to bowel function disorders . Guo Min [4] Shigella dysenteriae gavage manufacturing infected bowel dysfunction model, simulate human incidence of PI-IBS. Dendritic cell antigen-presenting cells in the main role in the start of the intestinal mucosal immune response gradually by the attention. The experimental by bowel dysfunction rat model of infection is established, the observation of dendritic cells in the intestinal tissue and the expression of cytokines, to explore changes in rat intestinal immune tolerance. Purpose through the creation of a the bowel dysfunction rat model of infection, intestinal tissue dendritic cells (dendriticcells, DCs) In this study, rats were observed in the expression and maturation of the intestinal mucosa, and detection of cytokines in peripheral blood and intestinal tissue IL-1β, IL-4 expression of the case, the difference between the ileum and colon in rats intestinal immune tolerance function changes. 60 male Wistar rats were randomly divided into control and experimental groups, with the Shigella dysenteriae gavage manufacturing bowel dysfunction rat model after infection. The experimental group application concentration the 9 × 108CFU/ml Shigella dysenteriae 1 ml gavage acute intestinal infection caused by normal control group with normal saline orally. Stool consistency was observed after infection, distal colon pathological examination at 1, 4, 7, 10, 13-day trip stool culture, and at 16, 19, 22, 25 days rectal balloon dilation measured intestinal capacity sensory thresholds. Modeling success, were evaluated in rat distal ileum and distal colon tissue gross morphology and histology score Immunohistochemical detection of intestinal tissue surface molecules CD11c costimulatory molecules CD80, CD86 expression, detection of cytokines IL-1β, IL- 4 expression and ELISA method peripheral blood cytokines IL-1β, IL-4 expression. The experimental group rats after infection 16 to 22 days stool changes, the intestinal sensory threshold than the normal control group was significantly decreased (P lt; 0 .05), and the stool culture of Shigella dysenteriae (-) , histology, back to normal. Gross morphological and histological scores with the control group of rats terminal ileum and distal colon tissue, there was no significant difference in sex (P gt; 0.05); immunohistochemistry tips experimental rats terminal ileum CD11c, CD80, CD86 expression There was no significant difference between the control group sex (P gt; 0.05); distal colon of CD11c, CD80, CD86 expression were significantly higher than the control group (P lt; 0.05). Compared with the control group, the experimental rats terminal ileum and distal colon IL-1β expression were significantly increased (P lt; 0.05), IL-4 expression compared with control group no significant difference in sex (P gt; 0.05). IL-1β expression of cytokines in peripheral blood of experimental rats compared with the control group tended to increase, but no significant difference in sex (P gt; 0.05); significantly lower (P lt; 0.05) compared with the control group of the cytokines IL-4 expression. Conclusion (1) Shigella flexneri Shigella the gavage method successfully established animal models of bowel dysfunction in rats infected. (2) after infection, bowel dysfunction rat peripheral IL-1β expression tended to increase compared with the control group, while IL-4 expression was significantly reduced peripheral immunomodulatory dysfunction in rats; (3) experimental rats terminal ileum and distal colon IL-1β expression were significantly increased, while no significant increase in IL-4 expression rat intestinal tissue presence of a mild inflammatory reaction; (4) the experimental group rat distal colon of CD11c, CD80 and CD86 was significantly increased, while no significant change in the terminal ileum, rat distal colon tissue dendritic cells tend to differentiation, development, maturation, local immune tolerance functions to reduce distal colon, promote the occurrence of immune response, affecting the gut pro-inflammatory cytokines and anti - inflammatory cytokines expression imbalance in the intestinal disorder.

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