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Objective: To study tumor-bearing mice spleen and peripheral blood myeloid suppression proportion of cells (myeloid-derived suppressor cells, MDSCs) changes in the organization of the distribution, its with serum nitric oxide (nitric oxide, NO) The level of the relationship, to explore both the role and significance of liver cancer immune escape. Method: using hepatoma cells using orthotopic implantation of orthotopic liver cancer model in mice, by flow cytometry in normal mice and the Netherlands tumor-bearing mice 7d, 14d, 21d spleen and peripheral blood MDSCs ratio; immunohistochemical staining MDSCs in spleen tissue distribution the positioning analysis; nitrate reductase assay serum NO content. Results: 1, Netherlands tumor-bearing mice 7d, 14d, 21d spleen MDSCs accounted for the proportion of mononuclear cells, respectively (6.07 ± 1.82)%, (11.07 ± 2.03)%, (16.49 ± 3.18)%, than in normal mice ( (4.11 ± 1.06)%) was significantly higher (P lt; 0.05), and extended with the tumor-bearing time and gradually increase the proportion of MDSCs in. Normal mouse spleen the little the MDSCs the content, scattered distribution in the red pulp. Lymphatic sheath tumor-bearing, a large number of MDSCs gathered around the small arteries in the marginal zone and white pulp area. 2, tumor-bearing mice 7d, 14d, 21d peripheral blood MDSCs proportion of the total mononuclear cells were (8.65 ± 1.01)%, (14.20 ± 2.52)%, (27.51 ± 2.98)%, than in normal controls ((2.51 ± 0.44)%) was significantly higher (P lt; 0.05), and tended to increase with the tumor-bearing time (P lt; 0.05). 3, the Netherlands tumor-bearing mice 7d, 14d, 21d serum in NO content were (72.02 ± 5.05) μmol / L, (94.70 ± 3.34) μmol / L, (78.90 ± 8.19) μmol / L, serum than in normal mice NO content ((57.03 ± 16.94) μmol / L significantly increased, the difference was statistically significant (P lt; 0.05) 4 MDSCs ratio and serum levels of NO in the peripheral blood of tumor-bearing mice were positively correlated (r = 0.869 , P lt; 0.01). Conclusions: 1, the proportion of the total mononuclear cells of tumor-bearing mice spleen MDSCs was significantly higher than in normal mice, gradually increased with the extension of the time of tumor-bearing and MDSCs gathered in tumor-bearing mice spleen in the thymus-dependent areas, the number of prompts MDSCs associated with tumor progression, MDSCs can interact with T lymphocytes affect immune function of T lymphocytes inhibit the anti-tumor effect, promoting the growth of tumor cells. 2, tumor-bearing mice the proportion of MDSCs in peripheral blood and serum NO levels tended to increase with the tumor-bearing time, both were positively correlated, suggesting that the play immunosuppressive effects of MDSCs through NO synthesis is involved in tumor immune escape, and promote the growth of tumor cells.
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