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Human Augmenter of Liver Regeneration and Evaluation of Its Bioactivity

Author: ChenZuo
Tutor: YouSong；KongXiangPing
School: Shenyang Pharmaceutical University
Course: Microbial and Biochemical Pharmacy
Keywords: ALR Gene therapy Plasmid RT-PCR
CLC: R575
Type: Master's thesis
Year: 2009
Downloads: 8
Quote: 0
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Abstract


Augmenter of liver regeneration the the Factors ( human augmenter of liver regeneration , The hALR is ) cloned from human fetal liver tissue to a new kind of promoting hepatocyte proliferation factor , in addition to the promotion of hepatocyte proliferation , but also protect the liver , and the prevention of liver fibrosis . The purpose of this experiment is to clone to liver regeneration enhanced factor ALR205 gene and study its role in the treatment of acute liver injury . The method by RT-PCR technology coding region was amplified from HepG2 cells ALR205 constructed the eukaryotic expression vector pCDNA3.1- ALR205 . The the recombinant plasmid 200μg/kg dose tail vein injection given CCL4 induced acute liver injury in rats , serum AST , ALT level , liver histopathological changes in liver tissue of proliferating cell nuclear antigen (Proliferating cell nuclear antigen PCNA) and control pCDNA3.1 -ALR125 gene therapy to observe ALR205 gene efficacy of acute liver injury . Combined with RT-PCR was used to detect the liver tissue ALR205 mRNA expression . The results of the the cloned ALR205 gene sequencing results consistent with those reported . Two recombinant plasmids for acute liver injury in rats , serum enzyme inspection results show that the peripheral blood AST ( aspartate aminotransferase ) , ALT ( alanine aminotransferase ) levels were significantly lower ( P lt; 0.01 ) , liver pathology damage dramatically reduced liver PCNA index , treatment group were significantly higher than the other groups ( P lt ; 0.01 ) and ALR205 than ALR125 , promote hepatocyte proliferation in a significant difference ( P lt; 0.05 ) , only ALR205 gene therapy group detected positive bands . Conclusions. ALR205 recombinant plasmid expression product in vivo by promoting the proliferation of liver cells , reducing serum AST , ALT level of play against acute liver injury .

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CLC: > Medicine, health > Internal Medicine > Digestive and abdominal diseases > Liver and gall bladder disease
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