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Prostatitis the male urogenital common data show that about 50% of men in their lifetime had prostatitis symptoms. Chronic prostatitis is the most common type of prostatitis in China, accounting for 33% of the urology outpatient. Its complex etiology, pathogenesis is unknown, there is widespread controversy; recent studies show that immunological factors may provide a major pathogenesis. Chinese Urological Association prostate diagnosis and treatment guideline, non-steroidal anti-inflammatory analgesics are recommended as empirical medication for chronic prostatitis. Clinical studies, can ease the symptoms of chronic prostatitis, lower NIH-CPSI score. The purpose of this study is to establish the mechanism of chronic prostatitis animal model based on the concept Chase celecoxib, and to explore its pharmacological effects and possible inflammation. IL-8 expression, cellular and molecular changes observed at the same time, to further explore the possible pathological mechanisms of chronic prostatitis and celecoxib in which the mechanism. Objective: by copy of rats with chronic prostatitis model, looking for a good experimental platform for clinical research. Materials and Methods: 40 male Wistar rats, weighing 200-230g, 7 weeks old, were randomly divided into four groups A, B, c, D, n = 10. Group A was the normal control group, the remaining three groups inflammation. The inflammation group underwent surgery on the left side of the prostate ventral injection of saline 0.1 ml of 1% carrageenan rat chronic non-bacterial prostatitis model; normal group underwent surgery at the left side of the prostate ventral injection of saline 0.1ml As a control group. B and group C rats were feeding 3 days and 7 days after the lien of specimens for testing, group A and group D 4 weeks after the feeding. The rat prostate dirty / body ratio and histopathological changes. ① prostate dirty / body ratio: the B rats Prostate dirty / body ratio was 1.46 ± 0.09mg / g, the C group was 1.45 ± 0.07mg / g, D group 1.37 ± 0.09mg / g, the control group was 1.06 ± 0.08mg / g; inflammation in the modeling group, the prostate tissue wet weight increased significantly, with group A (normal control group) compared with the significant difference (P lt; 0.05) (2) of prostate pathological changes: group B in interstitial edema and inflammatory cell infiltration, the glandular secretion bad, the significantly worse than group A (P lt; 0.05), fibrous tissue, no significant difference (P gt; 0.05). Group C and Group D in interstitial edema, inflammatory cell infiltration, poor glandular secretion and fibrous tissue, than those in group A was significantly increased (P lt; 0.05). Conclusion: the rat chronic prostatitis model to build a successful purpose: To observe the Chase celecoxib treatment of rats with chronic non-bacterial prostatitis cytokines IL-8 expression changes in prostate tissue pathology, and to explore the possible mechanism. Materials and Methods: The choice of experimental animals, rats with chronic prostatitis model prepared in the control group during the procedure with reference to the first part. Then 10 rats as normal control group, group A. Another 30 rats were established model of inflammation. The 7th day after the surgery, 30 inflammatory rats were randomly divided into group B, C, D, B group, model control group, C group celecoxib experimental low-dose group, D group celecoxib experiment high-dose group. According to the following scheme gavage once a day for 4 weeks. (A group: the saline 0.5ml/100g weight; and B: saline 0.5ml/100g weight; Group C: the celecoxib 2mg/100g weight; Group D: plug celebrex cloth 4mg/100g weight.) 4 weeks after indwelling specimens, observed changes in body weight gain in rats before and after administration, prostate dirty / body ratio, prostate tissue pathological changes and serum IL-8 level changes. Explore celecoxib possible mechanism of action. Changes in weight gain in rats: Group B rats weight gain slower weight gain was significantly less than in group A (P lt; 0.05) after 4 weeks; celecoxib experimental group, compared with the c group D, weight gain Group B faster, significantly different (P lt; 0.05), as compared to the group A, no significant difference (P GT; 0.05) (2) prostate dirty / Body ratio: B group (model control group) in rat prostate tissue wet weight increased significantly compared with the other groups have a very significant difference (P lt; 0.05); C group and D group (celecoxib cloth low-dose and high-dose group) compared with the control group, the difference was not significant (P> 0.05) (3) prostate pathological changes: C group and D group, interstitial edema, inflammatory cell infiltration, poor glandular secretion and fiber tissue, compared with group B were significantly reduced, the difference was significant (P lt; 0.05) Group C and Group D, the difference was not significant (P> 0.05). (4) serum IL-8 was measured: B rats serum IL-8 level in group A, c group, compared to the D group were significantly higher, the difference was significant (P lt; 0.05); c group celecoxib low-dose group and D group celecoxib between serum IL-8 levels in the high dose group of celecoxib, the difference was not significant (P> 0.05); group C and group D serum IL-8 level with the A group and the control group compared, the difference was not significant (P gt; 0.05). Conclusion: ① celecoxib for the treatment of chronic prostatitis in rats. (2) IL-8 has a certain diagnostic value for chronic prostatitis, is expected to become a secondary diagnosis of chronic prostatitis indicators. ③ celecoxib may inhibit the inflammatory response and immune regulation, to the treatment of chronic prostatitis improve symptoms and inhibit lesion.
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