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Novel Drug Carrier Systems Based on Nano Hydroxyapatite Materials

Author: MaHongMei
Tutor: JiaNengQin
School: Shanghai Normal University
Course: Physical and chemical
Keywords: Inorganic nanoparticles Calcium phosphate nanoparticles Drug-loaded carrier Antisense oligonucleotide Multifunctional magnetic contrast agent composite particles
CLC: TB383.1
Type: Master's thesis
Year: 2011
Downloads: 110
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Abstract

With the development of modern medical technology and materials science, especially inorganic nanoparticles to biomolecules drug diversity and potential applications have become the forefront of the field of materials science. The new inorganic inorganic nano-particles such as gold nanoparticles, silica nanoparticles, calcium phosphate nanoparticles as gene medicines carrier becoming a hotspot. Among them, calcium phosphate nano-particles unique biocompatible, bioabsorbable, biodegradable, low toxicity and chemical stability so that it can be effectively introduced into a cell, while the calcium phosphate particles to adhere and delivery of biologically active macromolecules drugs (such as proteins, antibodies, nucleic acids, contrast agent) can enter as a drug carrier to human tissue damage or lesion, and the calcium phosphate as a pharmaceutical carrier possible. The calcium phosphate carrier to form a chemical bond to the calcium ions and the antisense nucleic acid structure of the phosphate backbone groups to achieve protection and transfection of nucleic acids, antisense nucleic acid can be by the pharmaceutical carrier load drugs selectively concentrated in cancerous portion to reduce degradation and loss drugs, to reduce cytotoxic drugs damage normal tissue, while improving drug bioavailability. This paper utilization microemulsion method to prepare antisense oligonucleotide - calcium phosphate nanoparticle gene drug delivery system, the antisense oligonucleotide doped calcium phosphate lattice into HeLa cells to achieve effective transfected, as Construction of the new antitumor drug carrier systems and to improve the the antisense drugs utilization efficiency as a main direction of the research. Further compound the magnetic contrast agents to hydroxyapatite prepared fluorescently labeled contrast function multifunctional nano-particles to provide research-based for multifunctional drug contrast agent development. The paper consists of three parts, and its main contents are as follows: The first part focuses on the use of water-phase synthesis prepared nanoscale hydroxyapatite. Water phase synthesis the nanoscale spherical hydroxyapatite and study the cytology effect in HeLa cells, the nanoscale spherical hydroxyapatite by MTT assay to detect cytotoxic experiments show that in certain hydroxyapatite within the concentration range of the cell has a good biological safety. The second part of the antisense nucleic acid - hydroxyapatite nano-particle drug loading system. As the anticancer drug ASODNs pharmaceutical carrier hydroxyapatite, i.e. use of the calcium ions can react with the phosphate groups on the ASODNs of doping method successfully prepared the compound ASODNs-hydroxyapatite nanoparticles with UV-visible spectroscopy, fluorescence spectroscopy, transmission electron microscopy (TEM), MTT method, flow cytometry and other detection methods to confirm the success of the drug carrier build higher transfection efficiency and can effectively improve the anti-cancer efficiency of anti-cancer drugs. With a laser confocal microscope, biological electron microscopy confirmed the composite drug carrier into cells promote apoptosis of tumor cells to be truly effective. Third part explored the preparation and characterization of the compound manganese contrast agent-ASODNs-hydroxyapatite, transmission electron microscopy (TEM), vibrating sample magnetometer and laser scanning confocal microscopy and other methods to detect, the results showed that the more successful prepared having a functional magnetic resonance imaging targeted contrast agent has an anticancer efficacy of the compound dispersibility of the nanoparticles, which have a higher fluorescent effect of the relaxation rate, the exploration and study novel multifunctional gene drugs The development of new methods and new ideas.

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