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Objective: To explore the adipose-derived stem cells (adipose-derived stem cell, ADSC) transplantation on cerebral ischemia in rats nerve axon regeneration mechanism, by observing the ADSC transplantation on cerebral ischemia rat brain tissue Neurocan ('neural proteoglycans ), GFAP (glial acidic protein), Neuritin (neurite protein), NF200 (neurofilament protein 200) expression, to provide experimental evidence for ADSC transplantation in the treatment of cerebral ischemic diseases and its mechanism. Methods: 1, cultured in vitro the ADSC, immunofluorescence surface markers. Animal groups: 54 Clean SD rats were randomly divided into sham operation group (Sham group), middle cerebral artery occlusion (MCAO group) and MCAO the ADSC treatment group (ADSC group). Each group was divided 7d, 14d, 28d three subgroups, each subgroup 6. 3 Reference modified Zea-Longa thread embolism of middle cerebral artery embolization (middle cerebral artery occlusion, MCAO) model of focal cerebral ischemia. 4, DAPI (4 ',6-diamidino-2-phenylindole 2hci) in vitro markers ADSC. ADSC transplantation: ADSC group in the successful modeling ADSC (containing 1 × 106 cells) 1d injected into the left ventricle. 6 rats in each group at different time points before and after transplantation nerve function mNSS score. , Drawing and detection of 7.1 postoperative 7d, 14d, 28d rat brain perfusion, drawing, sliced. 7.2 observed by fluorescence microscopy of DAPI labeled ADSC survive in the body. 7.3 Western blot method detection GFAP, Neurocan, Neuritin, NF-200 protein. 7.4 immunofluorescence staining GFAP, Neurocan, Neuritin, NF-200 antigen expression. 7.5 H-E staining observed pathological findings. 8, statistical analysis of results: 1 of brain tissue in the the ADSC group around the infarct striatum and frontal and parietal cortex labeled with DAPI-positive cells can be observed. ADSC transplantation can reduce the extent of damage of nerve function. HE staining results showed that a large nerve cells of the infarct necrosis, proliferation of glial cells of the surrounding area, glial scar formation. 4, Western blot method analysis showed that ADSC group cerebral ischemia surrounding area organizations at each time point MCAO group compared of GFAP, Neurocan expression significantly reduced Neuritin, NF-200 expression was significantly increased, and the difference was statistically significant (P lt; 0.05). Conclusion: ADSC transplantation can cause cerebral ischemia Neuritin NF-200 effective expression and inhibition of GFAP-positive cells, Neurocan in the expression, and promote axonal regeneration and repair.
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