|
This issue through application EliVision TM Plus immunohistochemistry (immunohistochemistry, IHC) detection of colorectal cancer (colorectal cancer, CRC) organization Twist and E-cadherin and β-catenin and by in situ hybridization to detect colorectal cancer in E-cadherin expression, explore the following questions: To investigate the Twist, E-cadherin and β-catenin expression in adenomas and colorectal cancer tissues and normal colorectal tissue, colorectal clinical pathological features; explore Twist relationship; investigate the E-cadherin mRNA in colorectal normal tissue of colorectal adenoma and colorectal cancer tissue expression of E-cadherin and β-catenin expression in colorectal cancer tissues and relationship with clinical and pathological features of patients; consistency of E-cadherin expression in both immunohistochemistry and in situ hybridization to detect colorectal cancer tissues. Collection PLA 251 Hospital Pathology colorectal cancer from January 2004 to June 2004, 60 cases of postoperative specimens, as well as 20 cases of colorectal adenomas and cancer, 20 cases of adjacent normal mucosa, EliVision TM Plus Immunohistochemistry was used to detect colorectal cancer tissues Twist, E-cadherin and β-catenin expression, another expression of E-cadherin in situ hybridization to detect colorectal cancer tissues; analysis of three indicators relationship as well as the expression of the relationship between the three and the patient's age, gender, tumor size, degree of differentiation, TNM stage, depth of invasion, and lymph node metastasis; analysis of the consistency of the two methods for detecting the expression of E-cadherin. Twist the positive rate of E-cadherin and β-catenin in 60 patients with colorectal cancer specimens were: 53.3% (34/60), 35% (21/60), 18.3% (11/60); 20 cases The positive rate of colorectal adenoma specimens were: 10% (2/20), 70% (14/20), 65% (13/20); positive rates were normal mucosa specimens in 20 paracancerous : 0% (0/20), 100% (20/20), 100% (20/20). Twist in the positive rate of colorectal cancer tissues was significantly higher than the positive rate of normal mucosa adjacent to colorectal adenomas and carcinomas, E-cadherin and β-catenin in the positive rate of colorectal cancer tissues was significantly lower than that of colorectal adenomas and The positive rate of cancer adjacent normal mucosa, a statistically significant difference. Colorectal adenomas E-cadherin and β-catenin positive rate lower than the normal rate of mucosal tissues, a statistically significant difference; Twist expression rate was higher than normal tissue, but the difference in colorectal adenomas No statistically significant. Twist in colorectal cancer abnormal high expression of E-cadherin and β-catenin expression and tumor differentiation degree, TNM stage, depth of invasion, lymph node metastasis, and 5-year survival rate is closely related to, but not with age, gender and tumor size clinicopathological features. Twist and E-cadherin, β-catenin positive expression in 60 cases of colorectal cancer tissues were 34 cases, 21 cases, 11 cases, seven cases in positive Twist expression in 34 cases of E-cadherin expression, ectopic expression of β-catenin Spearman correlation analysis, the results showed: Twist and E-cadherin expression was significantly negatively correlated; Twist and β-catenin expression was both a significant negative correlation; between the expression of E-cadherin and β-catenin was significantly positively related to . In tissue samples of 60 patients with colorectal cancer, E-cadherin mRNA positive rate was 38.3% (23/60), 23 cases of E-cadherin mRNA in positive samples positive for E-cadherin protein 21 cases; 20 cases of colorectal adenomas tissue samples, E-cadherin mRNA-positive rate of 75% (15/20) in 15 cases of E-cadherin mRNA in positive samples, E-cadherin protein was positive in 14 cases; 20 cases of normal colorectal mucosa specimens, E- cadherin mRNA-positive rate was 100%. The positive rate of E-cadherin mRNA in colorectal cancer tissues was significantly lower than the adjacent normal mucosa of colorectal adenomas and carcinomas positive rate, a statistically significant difference. Colorectal adenomas E-cadherin mRNA positive expression rate higher than that in normal mucosa, a statistically significant difference; abnormal expression of E-cadherin mRNA and the degree of tumor differentiation, TNM stage, depth of invasion, lymph node metastasis and 5 year survival rates are closely related, while no significant relationship with age, sex, and tumor size clinicopathological features. Twist expression and the occurrence of colorectal adenomas relationship is not close, Twist may not be involved in the formation of colorectal adenomas; E-cadherin has a high degree of consistency in the immunohistochemistry and in situ hybridization are two ways to detect; Twist E-cadherin and β-catenin to participate in the process of taking early metastasis cancer, colorectal cancer invasion and early metastasis important biological indicators, joint detection Twist, E-cadherin and β-catenin early metastasis and prognosis prediction of tumor important, Twist in the future may become a new target for colorectal cancer targeted therapy.
|