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Objective To observe the the kidneys remote post-processing (RI-Post), ATP after treatment (ATP-Post) and the combination on myocardial ischemia-reperfusion injury in rats. 40 Methods Male SD (Sprague-Dawley) rats, weighing 250 to 300 g, were randomly divided into five groups: (1) sham-operated (Sham) group (n = 8); ② The ischemia-reperfusion (I / R) group 4 RI-Post group (n = 8); the ⑤ joint (Joint) group (n = 8) (n = 8); (3) ATP-Post group (n = 8); Anterior descending branch of the left coronary artery ligation in rats prepared myocardial ischemia-reperfusion model. The sham group threading ligation observed 150min; ischemia-reperfusion groups: ischemia 30min, reperfusion 120min; the ATP-Post group: ischemia for 30 min reperfusion 120 min reperfusion before the start of 5min tail vein pump into ATP ( 0.4mg/kg.min); RI-Post group with ischemia-reperfusion group operations, abdominal surgery to find kidney, separation of the renal artery and renal nerves, renal artery threading folder arteries before reperfusion repeated occlusion of the left renal artery (blocked for 30 seconds, 30 seconds recanalization, repeated three times), reperfusion 120min; Joint group: kidney remote ischemic post-processing treatment drugs. Record II-lead ECG. The end of the experiment serum superoxide dismutase (SOD), malondialdehyde (MDA); creatine kinase isoenzyme (CK-MB) content; HE staining under light microscopy observation of myocardial tissue morphology; immunohistochemistry of apoptosis-related gene bcl-2 and bax protein expression. Results (1) serum SOD, MDA and CK-MB content: Sham group (88.59 ± 3.66 VS 73.97 ± 3.36), less than the I / R group SOD MDA (3.32 ± 0.40 VS 6.46 ± 0.43) and CK-MB (644.8 ± 113.7 VS 1210.4 ± 108.3), the difference was significant (P lt; 0.05); with the I / R group than the RI-Post group, the increase in ATP-Post Group and Joint group SOD (73.97 ± 3.36 VS 79.11 ± 2.54,78.79 ± 2.08,83.95 ± 3.04), MDA (6.46 ± 0.43 VS 4.63 ± 0.44,4.50 ± 0.37,4.03 ± 0.31) and CK-MB (1210.4 ± 108.3,796.5 ± 139.7,786.9 ± 168.4,718.4 ± 118.1) reduced the difference significant sex (P gt; 0.05); RI-Post group and of ATP-Post group than SOD, of MDA and CK-MB difference without significantly with sex (P gt; 0.05); RI-Post group and of ATP-Post group than the Joint The increase in group SOD, MDA and CK-MB reduced, the difference was significant (P lt; 0.05). (2) HE staining under light microscope myocardial morphological changes: sham group cell interstitial edema, visible muscle fibers arranged in neat rows, cell boundaries are clear, no granulocyte infiltration, erythrocyte leakage. Myocardial cells of the I / R group interstitial edema, severe swelling of the muscle fibers, ill-defined cell stripes disappear seen more myeloid spotty infiltration, and can be seen a small amount of red blood cells leak. The RI-Post group, ATP-Post group and Joint myocardial cells with mild edema, clear boundaries, the myocardial cells arranged relative rules, granulocyte infiltration less rare red blood cell leakage. (3) myocardial tissue apoptosis relevant factor Bax and Bcl-2 expression: Sham group myocardial cells with a small amount of Bax, Bcl-2 protein expression. I / R group, RI-Post group, ATP-Post Group and Joint group and Sham group of Bax and Bcl-2 protein expression increased (P lt; 0.05); the RI-Post group, ATP-Post Group and the Joint group and I / R group increased significantly compared to the Bcl-2 protein expression and Bcl-2/Bax value of Bax protein was significantly reduced (P lt; 0.05); compared to the RI-Post group, ATP-Post group of Bax, Bcl-2 protein expression the difference was not statistically significant (P gt; 0.05); the Joint group RI-Post Group and the ATP-Post group compared to the expression of Bcl-2 protein expression, Bcl-2/Bax value increased significantly, and Bax protein significantly reduced (P lt; 0.05). Conclusion ATP treatment attenuate myocardial ischemia-reperfusion injury; kidney remote ischemic post-processing has a role in reducing myocardial ischemia-reperfusion injury; their combination to further reduce myocardial ischemia-reperfusion injury.
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