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In this experiment, the casein as a protein source, fish oil as a source of fat, dextrin as a sugar source, respectively prepared containing different concentrations of vitamin B 1 , B 2 and B 6 semi-purified diet, feeding in the hydrostatic system Chinese mitten crab 10 weeks, growth performance, feed efficiency, and related enzyme activity and body tissue biochemical composition of the indicators discussed vitamin to B 1 B 2 and B 6 on the physiological functions of the Chinese mitten crab, the Chinese mitten crab on polyline regression calculation dietary vitamin B 1 B 2 and B 6 appropriate demand. Test specific content and the results are as follows: 1 Chinese mitten crab studies of dietary vitamin B 1 appropriate demand the Chinese mitten crab the vitamin B feed < / sub> appropriate demand. Test casein as the protein source, preparation of vitamin B 1 content of the semi-purified diet of 0,10,20,40,80,160 and 320mg/kg of the seven kinds of nitrogen, etc., weight at 10 weeks of 1.32 ± 0.15g Chinese mitten crab feeding in a hydrostatic system. The results showed that dietary vitamin B 1 of mitten crab survival rate, body crude fat and ash content of no significant impact, but can significantly improve the crab weight gain, protein efficiency, crude vitamin B 1 content, protein content and hepatopancreas and can significantly reduce the moisture content of feed conversion ratio and crab. When when the vitamin B feed dosage 80mg/kg, the crab weight gain and protein efficiency the highest, and the feed conversion ratio was significantly higher than the other groups (20mg/kg); The trend of the moisture content in vivo contrast, the lowest value in the test group of the vitamin B 1 dosage 80mg/kg. Vitamin B 1 dosage of 80 and 160mg/kg, crab crude protein content was significantly higher than the added amount of the lower (0,10,20 mg / kg) test group. Amylase activity in the hepatopancreas With vitamin B 1 add the amount of the rise was significantly higher vitamin dosage 80mg/kg test group, crab amylase activity was significantly higher than the vitamin B add less (0 10mg/kg) and add higher (160 and 320mg/kg) test group; trend of the lactic acid content in the hepatopancreas contrast the feed vitamin B 1 dosage 80mg/kg, lactic acid content in the crab hepatopancreas significant lower than that of other groups. Based on the weight gain of the Chinese mitten crab, the to vitamin B hepatopancreas 1 content and amylase, polyline regression method to get the Chinese mitten crab feed vitamin B 1 optimal demand for 55.08-66.71mg/kg. The Chinese mitten crab study of dietary vitamin B 2 appropriate demand the Chinese mitten crab dietary vitamin B 2 appropriate demand . Test casein as the protein source, and the preparation of vitamin B 2 contents were 0,3,6,12,24,48 and 96mg/kg of the seven kinds of nitrogen, etc. can be a semi-purified diet weight at 10 weeks of 1.32 ± 0.11g Chinese mitten crab feeding in a hydrostatic system. The results showed that the diet containing different levels of vitamin B 2 Chinese mitten crab survival rate, feed conversion ratio, protein efficiency ratio and body moisture, ash and crude protein content were not significantly affected, but With the vitamin B 2 content increased weight gain crab presents the trend first and then decreased when dietary vitamin B 2 add the amount reached 12mg / kg, the weight gain rate is the highest (201.15%), significantly higher than 0,3,48 and 96mg/kg 151.97% of the test group, 151.39%, 155.83% and 141.19%, respectively. Opposite trend crab trend of the crude fat content in the body weight gain rate, the lowest vitamin B 2 level of 12mg/kg. In addition, changes in dietary vitamin B 2 content does not significantly affect the the the crab hepatopancreas vitamin B 2 accumulation, but the lack of feed the vitamin B 2 < / sub> will significantly inhibit the vitality of the Chinese mitten crab hepatopancreas D-amino acid oxidase. According to the vitality of the D-amino acid oxidase in the rate of weight gain and hepatopancreas of Chinese mitten crab, polyline regression method calculated the Chinese mitten crab of vitamin B feed 2 suitable demand for 8.25-10.02 mg / kg. 3 vitamin B 2 Chinese mitten crab blood lipids and antioxidant capacity of vitamin B 2 Chinese mitten crab lipid peroxidation ability. Test casein as the protein source, and the preparation of vitamin B 2 contents were 0,3,6,12,24,48 and 96mg/kg of the seven kinds of nitrogen, etc. can be a semi-purified diet weight at 10 weeks of 1.32 ± 0.11g Chinese mitten crab feeding in a hydrostatic system. The results showed that the vitamin B 2 Chinese mitten crab serum triglyceride concentrations did not significantly affect serum cholesterol and low-density lipoprotein cholesterol content as vitamin B 2 added amount is increased significantly reduced. The dietary vitamin B 2 dosage 12, 24 and 48mg/kg, serum cholesterol content was significantly lower than the other test groups, low-density lipoprotein cholesterol was significantly lower than the vitamin B 2 dosage 0,3,96 mg / kg of the test group. Crab hepatopancreas glutathione reductase vitality as dietary vitamin B 2 add the amount of the increased significantly elevated, when the vitamin B feed 2 Add amount of 12mg/kg, its activity was significantly higher than the other test groups. The Chinese mitten crab the the vitamin B feed 6 suitable demand research suitable for the demand of the Chinese mitten crab of the feed vitamin B 6 . Test casein as the protein source, preparation of vitamin B 6 contents were semi refined of 0,10,30,60,100,150,200 and 400mg/kg of the eight kinds of nitrogen, etc. can feed, feeding body weight at 10 weeks of 2.12 ± 0.56g Chinese mitten crab in the hydrostatic system. The results showed that the the different dietary content of vitamin B 6 of mitten crab survival rate, weight gain, body moisture and ash content were not significantly affected, but dietary vitamin B 6 can significantly improve the juvenile crabs protein utilization, body crude protein and crude fat content. The the feed vitamin B 6 content increases from 0 to 100mg/kg, protein utilization along with significantly increased, but the vitamin B 6 add more than 100mg/kg when the protein utilization of the test group showed a downward trend. Similar to the trend of body crude protein and crude fat content and protein utilization, which vitamin B 6 dosage 100mg/kg processing group, crab, crude protein and crude fat content was significantly higher than the control the group and 400mg/kg vitamin B 6 Add Group. The hepatopancreas aspartate aminotransferase and alanine aminotransferase vitality first enhanced with increasing the amount of vitamin B 6 Add, add the amount of more than 100mg/kg, the activity of two enzymes decline add 100mg/kg vitamin B 6 test group, crab, alanine aminotransferase activity was significantly higher than the other test groups. Using polyline regression calculated according to each treatment group mitten crab feed protein utilization and hepatopancreas alanine aminotransferase activity, the Chinese mitten crab the the feeding light bucket vitamin B 6 The optimal demand 81.675-85.74mg/kg feed.
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