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Objective: To the erigeron extract can reduce transplant acute rejection reaction caused by pathological damage and prolong graft survival time. The the main observation Dengzhanhua extract of the study of chronic rejection and to explore its mechanism of action. Method: 1, microsurgical technique in rat by chronic renal allograft rejection in a rat model of F344 rats were used as donors, Lewis rats as recipients, implementation of rat orthotopic left renal transplant surgery, removal of the receptor right kidney. CsA 10d prevention of acute rejection, gavage, 5 mg / (kg · d). 2 kidney transplant in rats of different methods of administration are divided into four groups control group, immunosuppressants group, the erigeron low dose group and high dose group. Immunosuppressant group were the 10d from daily intraperitoneal injection of the mycophenolate mofetil 20mg/kg to 12 weeks after surgery, the low-dose group, high dose group over the same period, respectively, by intraperitoneal injection Dengzhanhua 5mg/kg and 10mg/kg of the extract solution The control group over the same period to the normal saline. 3, line serum creatinine and 24h urine protein content was measured after 8 and 12 weeks, respectively. 12 weeks after surgery serum vascular endothelial growth factor concentration in parallel transplant renal histological examination. 4, the data obtained are (?) The ± s SPSS13.0 statistical software for data analysis, the groups were compared using analysis of variance, P <0.05 Tip difference was statistically significant. Results: 1, 12 weeks after transplant renal histological examination pathological scores, the control group, low-dose group, high dose group and immunosuppressants group rated the basis Banff97 standard were 9.50 ± 0.93,8.38 ± 0.74,7.38 ± 0.74 , 5.75 ± 0.71. Low-dose group, high dose group than the, and immunosuppressants group rated in the control group (P <0.05) lower than that of the low-dose group (P <0.05), the high-dose group the immunosuppressants group rated immunosuppressant group rated lower than high-dose group (P <0.01). In after 4, 8 and 12 weeks of three-point-in-time detection, the high-dose group, immunosuppressants group at three time points serum creatinine were significantly lower than those in the control group (P <0.01), low dose group postoperative 8 weeks, 12 weeks, serum creatinine less than the control group (P <0.05). Immunosuppressant serum creatinine at three time points were significantly lower than that of the low-dose group (P <0.01), immunosuppressants in serum creatinine after 8 weeks, 12 weeks below the high-dose group (P <0.05). Serum creatinine in the high dose group after 8 weeks, 12 weeks less than the low-dose group (P <0.05). 3, after 24h urine protein levels in rats presented a rising trend over time. Immunosuppressant group 24h urine protein levels were lower than the control group (P <0.05) at all time points, the low-dose group 24h urinary protein levels lower than the control group at 12 weeks after surgery, high dose group 24h urine protein levels in the postoperative 8 weeks, 12 weeks were lower than the control group (P <0.05). The immunosuppressants group 24h urinary protein levels lower than the low-dose group after 8 weeks, 12 weeks, lower than the high dose group (P <0.05) after 12 weeks. 24h urine protein levels of the high-dose group compared to the lower dose group at all time points the difference was not statistically significant. 4, in the 12th week, the low-dose group, high dose group, the immunosuppressants serum vascular endothelial growth factor concentration is less than the control group, the low-dose group, high dose group of vascular endothelial growth factor concentrations were higher than the immunosuppressant the high dose group of vascular endothelial growth factor concentration is lower than the low dose group, the differences were statistically significant (P <0.01). Conclusion: 1, Dengzhanhua extract can alleviate chronic rejection of the transplant renal pathological damage and functional impairment, which can effectively reduce the rat chronic rejection lesions. The mycophenolate mofetil prevention and treatment of chronic renal allograft rejection better than Dengzhanhua extract. 2, Dengzhanhua extract may play its role in the protection of the transplanted kidney function and structure by reducing mechanisms such as vascular endothelial growth factor production.
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