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Objective: To compare different approach down the physical and chemical characteristics of antigen xenogeneic cancellous bone, screening out the appropriate antigen construct tissue engineered bone late to provide experimental basis. Methods: Adult sheep fresh vertebral cancellous portion, and prepared into a cylinder of a specific shape, the major axis and taken cancellous bone trabeculae direction, were randomly divided into A, B, C treatment group and control group, group D A group of materials using methanol / chloroform (1:1), 30% hydrogen peroxide and other chemical agents sequence defatted acellular deproteinized after calcined at 1000 ℃ 3H of the calciner, after simple treatment of the B group material line directly in the calcination furnace 1000 ° C calcination 3H, group C materials using only the group A chemical processing, D group only a simple treatment as a control, by porosimetry, histological examinations, X-ray diffraction analysis, X-ray Atomic Energy spectrum Elemental analysis, total nitrogen determination and universal mechanical tester detects compare different methods to deal with the physical and chemical properties of the samples. Results: three groups of samples of the groups of A, B, C, D are different degrees were kept natural network pore structure of the original bone tissue, scanning electron microscopy measurements macroporous pore size of 50-600μm, pore size is about 2gm, porosity of 55% -70%; X-ray (powder) diffraction shows the main component of the sample of the main component of the sample of A, B group hydroxyapatite (HAP) and a certain amount of β-tricalcium phosphate (β-TCP), C group hydroxyapatite ( of HAP); number of calcium and phosphorus atom of group A, B, C, D, respectively (1.647 ± 0.641): 1, (1.637 ± 0.096): 1, (1.774 ± 0.876): 1, (2.012 ± 0.278): 1, are close to the body of calcium and phosphorus than differences between groups were not statistically significant (P gt; 0.05); total nitrogen content of 0%, 0%, 4.43%, 4.06%: mechanical properties of A, B group than in C, D group (P lt; 0.05) AB between the two groups had no statistical difference (P gt; 0.05). Conclusion: treated by chemical or physical methods of cancellous bone dimensional space structure does not have a lot of damage, are reserved to the pore size range, the transport good connection between the surface of open pores and a high degree of porosity; through physical and calcination step can be more completely remove the antigen components of dissimilar cancellous bone of the decline in the mechanical properties of the material, and the simple use of chemical reagents soaked antigen component of the material can not be removed completely but its mechanical properties decrease little; calcining step after physical system go antigen xenogeneic bone scaffold into line with the requirements of bone tissue engineering scaffold material, but the cell compatibility and tissue compatibility still need further validation.
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