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Objective To investigate whether erythropoietin on cerebral ischemia in rat brain tissue protective effect, and to explore the frequency of cerebral ischemia erythropoietin administration, in order to more effectively protect cerebral ischemia in rat hippocampal neurons. improve behavioral defects. Methods 80 male SD (sprague-dawley, SD) rats were randomly divided into three groups: normal group, model group, the treatment group. In addition to the normal group, the other two groups were made of cerebral ischemia and reperfusion model; treatment group was divided into three subgroups among subgroups promote red element method of administration (intraperitoneal injection), the same dose (3000 IU / kg), only different dosing frequency three times administration group as ischemia to 1 immediately after ischemia 6h to 1 times, and after ischemia 12h to 1 times; 2 times administration group as ischemic immediate to 1, 6h after ischemia to 1; instantly to 1 1 dose group only ischemia. In cerebral ischemia after 24h behavior scores of rats in each group, are drawn in ischemic 24h, respectively, for HE staining pathological changes of immunohistochemical staining analysis of hippocampal neurons in B-cell lymphoma / leukemia - 2 gene (b-cell lymphoma gene 2, Bcl-2), B-cell lymphoma / leukemia 2 gene x protein (b-cell lymphoma gene 2 associated x protein, Bax) expression; addition hippocampus was determined by Western Blot half expression of cysteine ??aspartic protease -3 (cysteine-containing aspartatespecific proteases, Caspase-3). Results neurological behavioral score results, 2 dose group and 3-dose group compared with the model group, significant improvement (P lt; 0.05), the difference was statistically significant, compared to the single dose group and the model group little improvement, P gt; 0.05, the difference was not statistically significant; promote the expression of apoptotic protein Bax, three subgroups of the treatment group were far lower than the model group, P lt; 0.05, the difference was statistically significant; inhibitor of apoptosis protein Bcl 2 expression in the amount of 2 dose group compared with the model group was significantly higher P lt; 0.05, the difference was statistically significant; 1 dose group and three dose group model group, significantly lower, P lt ; 0.05, the difference was statistically significant; apoptosis terminal protein Caspase-3 expression, 2 dose groups in the treatment group and the 3-dose group was significantly lower than the model group, P lt; 0.05, the difference was statistically significance; 2 dose group Caspase-3 expression was significantly lower than the 3-dose group, P lt; 0.05, the difference was statistically significant; 1 dose group was significantly higher than that in the model group, P lt; 0.05 The difference was statistically significant. Conclusion The protective effect of erythropoietin on cerebral ischemia in rat brain tissue; erythropoietin cerebral ischemia in rats the neuroethology improve; erythropoietin able to protect hippocampal neurons by inhibiting apoptosis, erythropoietin through raised the Bcl-2 protein expression, at the same time lowered the expression of Bax protein to inhibit apoptosis. Erythropoietin can inhibit apoptosis terminal protein Caspase-3 expression; 2 dose group has a distinct advantage, cerebral ischemia in period 2 doses close to the best method of administration.
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