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Study on the in Vitro Caco-2 Cells Inhibiting Active Parts and the Quality Standard of Cynomorium Songarium Rupr
Author: ReXiDanMu·TuoHuTi
Tutor: ReNa·KaSiMu
School: Xinjiang Medical University
Course: Pharmacognosy
Keywords: Cynomorium songarium Rupr. Caco-2 chemical constituents determination
CLC: R284
Type: Master's thesis
Year: 2011
Downloads: 17
Quote: 0
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Abstract
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Objective:To investigate in vitro anti Caco-2 cells activities of different extraction parts from the Cynomorium songarium Rupr. and the chemical constituents there in contributable to anticancer activity. Methods:The inhibitory effects of these extraction parts towards Caco-2 cells were measured by MTT assay. The chemical compositions in the chloroform and ethyl acetate extraction parts were separated by column chromatography. Results:the IC50 values of the chloroform and the ethyl acetate extraction parts are 88.5μg/ml and 107.7μg/ml respectivly; 6 componds such as (+)-catechin (Ⅰ), choerospondin (Ⅱ), gallic acid (Ⅲ),β-Sitosterol (Ⅳ), ursolic acid (Ⅴ),β-Sitosterol-3-O-β-glucoside (Ⅵ) were isolated from the chloroform and the ethyl acetate extraction parts and their chemical structures were characterized by 1H-NMR and TLC (compared with the related reference substances). The content determined by using HPLC method forgallic acid and (+)-catechin were respectively 19.2% and 4%. Conclusion:Our results suggested that the extraction parts of Cynomorium songarium Rupr.have can inhibit Caco-2 cells, this research explained preliminarily that the Cynomorium songarium Rupr. possess anticancer activity. Furthermore, our works can provide a basic information for the progressive study of its anti-tumor function and quality standard.
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