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Background: Molecular Imaging vitro imaging detector disciplines of qualitative and quantitative research on live animals, the model system and the body's biological processes at the cellular and molecular level. In recent years, the development of molecular imaging technology quickly, making real-time small animal tumor models, non-invasive in vivo imaging has become possible. Vivo optical imaging of in vivo compared with the traditional method of detection has great advantages, called the field of molecular genetic testing technology revolution, it fluorescent protein into target cells or small animals by in vivo fluorescence imaging system in the body, non-invasive, dynamic observation of biological processes, this technology is extremely high detection sensitivity of tumor micrometastases, without involving radioactive substances, very safe, and has been widely used in cancer research. Red fluorescent protein (DsRed) as a reporter gene using liposomes mediated gene transfection method DsRed labeled mouse bladder cancer (BTT739) cells of the rat bladder cancer subcutaneous xenograft model the MAESTRO in vivo imaging system in vivo, non-invasive, dynamic study of the biological processes of the bladder tumor is the purpose of this experiment. Objective: To explore the red fluorescent protein (DsRed) labeled mouse bladder cancer growth and metastasis molecule fluorescence imaging features. Methods: Lipofectamine 2000 mediated gene transfection method, chickenβ-actin-DsRed-Neo vector transfected the BTT739 to mouse bladder cancer cell lines; after G418-screening, obtain a stable expression of DsRed monoclonal cell (BTT739-DsRed ); 615 mice 24 were randomly divided into three groups, hindlimb group 1 and 2 of the the hypodermic cell suspension, injection BTT739-DsRed cells, 3 injection BTT739 cells to establish xenograft model; the MAESTRO vivo imager settings excited optical wavelength 560-580nm, emission wavelength of 590-610nm, the exposure time 5000ms continuously observed for 4 weeks, the group 2 week mice were sacrificed and sectioned imaging, recording the fluorescent image of the tumor growth and metastasis, the tumor size, and the fluorescence signal value was measured; Statistical analysis of the relationship between the value of the tumor size and the fluorescence signal, systemic imaging and sectioned imaging. Results: 1. Successfully established the red fluorescent xenograft model of bladder cancer in mice. MAESTRO vivo imager excitation wavelength 560-580nm, emission wavelength of 590-610nm, exposure time 5000ms continuous observation of four weeks, one week observed hair red fluorescent tumor observed four weeks of lymph node metastasis, no far at the transfer. 3 1 for four weeks in a row determination of tumor fluorescence signal values ??as follows: 88.85 ± 17.65,122.26 ± 54.63,133.12 ± 69.06,714.58 ± 342.88counts; tumor size as follows: 12.78 ± 4.14,45.07 ± 21.71,82.55 ± 28.98 253.01 ± 67.27mm2; second set of body imaging tumor size of four weeks as follows: 11.63 ± 3.27,50.07 ± 23.41,89.76 ± 29.12,289.64 ± 73.56mm2, cut open the imaged sequentially: 12.24 ± 4.53,72.08 ± 30.12,141.09 ± 43.26 , 523.89 ± 236.78 mm24. statistical analysis of tumor size and fluorescence signal strength of a positive linear correlation (r = 0.74, t = 3.97, P lt; 0.05), whole body imaging with cutaway imaging tumor size had a positive linear related (r = 0.97, t = 10.00, P lt; 0.05), tumor size measured whole body imaging imaging is cut open after 70.85 ± 17.13%. Conclusion: murine bladder cancer xenograft model red fluorescence can be intuitive, continuous, sensitive observation of tumor growth and metastasis, fluorescent range also increases with increasing tumor fluorescence disappears, tumor necrosis, tumor metastasis after red The fluorescent expression be transferred.
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