|
Objective:Analysis the expression of cytokeratin 5/6(CK5/6) and epidermal growth factor receptor(EGFR)in breast cancer tissues.To investigate their distribution characteristic in the breast cancer and its relationship with the breast cancer histological grade,tumor size,axillary lymph node metastasis,estrogen and progesterone receptors.Provide an important basis on diagnosis,clinical treatment and prognosis of the breast cancer.Methods:Material taking and hematoxylin and eosin(HE) staining:Take the breast cancer tissue in 30 minutes after the modified radical mastectomy.Keep them in-80℃refrigerator cold storage.After paraffin-embedded,make the tissue into 3 microns sections.hematoxylin and eosin stain.Detect the expression of CK5/6 in 175 cases of infitrating ductal carcinoma and EGFR in 42 cases of breast cancer in the method of immunohistochemistry.Analysis the expression of the CK5/6 and EGFR in breast cancer tissue. Investigate the relationship between the expression of CK5/6 and EGFR and patient’s age,histological grade,tumor size,axillary lymph node status and the clinicopathologic parameters relationship.Results:l.The positive rate of CK5/6 is 17.7% in 175 cases of breast cancer tissues,the negative rate is 82.3%.The positive rate of EGFR is 4.8% in 42 cases of breast cancer tissues,the negative rate is 95.2%.2.The relationship between the expression of CK5/6 and menopause,tumor size,pathological grade,lymph node metastasis is not statistically significant(p>0.05), but the comparation of axillary lymph node metastasis is less than 4 and greater than or equal to 4 is significant difference(p<0.05).The relationship between the expression of EGFR menopause,tumor size,lymph node metastasis,pathological gradeⅡandⅢis not statistically significant(p>0.05),but the comparation of pathological grade I and II, axillary lymph node metastasis is less than 4 and greater than or equal to 4 are significant differences(p<0.05).3.The comparison between CK5/6 expression and the ER,PR results is statistical difference(p<0.01).In the patients of ER(-),PR(-),the CK5/6 positive expression rate is high.There is no significant difference(p>0.05) in the comparison among HER2(-), HER2(+) and HER2(++) and the comparison among HER2(+),HER2(++)and HER2 (+++).However there are significant differences(p<0.05) in the comparison between HER2(-) and HER2(+++),and the comparison between HER2(++) and HER2(+++). There is no difference(p>0.05) in the comparison between EGFR expression and ER, PR results,In the patients of ER(-),PR(-),the EGFR expression was lower,There is no significantly difference(p>0.05) in comparison between the patients with HER2(-) and HER2(+),HER2(+) and HER2(+++),HER2(++) and HER2(+++).however,there is statistical difference(p<0.05) in the comparison among HER2(-) and HER2(++), HER2(+++),HER2(+) and HER2(++).4.The CK5/6 expression rate in 23 cases of ER,PR and HER2 negative (triple-negative) breast cancer is 65.2%,the rate in 152 cases of patients with non three negative is 10.7%.the difference between the two is clear statistical significance (p<0.01),Conclusion:1.The expressions of CK5/6 and EGFR in breast cancer tissue are not related to menopause,tumor size,pathological grade,lymph node status.2.There are high positive rates of CK5/6 in ER and PR negative breast cancer,but the expressions of EGFR are not related to ER and PR status. The expressions of CK5/6 and EGFR in HER2 high positive breasr cancer are more than in the HER2 negative breast cancer.That suggest the expression of CK5/6 and EGFR are correlate to the invasion,metastasis and prognosis of breast cancer.3.The expressions of CK5/6 in breast cancer are not related to lymph node status,but there are high positive rates in more axillary lymph node metastasis.4.The positive rate of CK5/6 expression in triple-negative breast cancer is significantly higher than non triple-negative breast cancer,that can make molecular typing more accuratly,and direct the clinical treatment and predict prognosis.
|