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The tertiary structure of the hepatitis C virus internal ribosome entry site

Author: NingJunHong
Tutor: Alastair Murchie
School: Fudan University
Course: Medicinal Chemistry
Keywords: Hepatitis C virus (HCV) Internal ribosome entry site ( IRES ) Tertiary structure of the study Ion concentration RNA concentration Temperature
CLC: R373
Type: Master's thesis
Year: 2008
Downloads: 47
Quote: 0
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Abstract


Hepatitis C virus (hepatitis C virus) genome is a single-stranded RNA of about 9.5 kb , and it encodes a protein of 3000 amino acids ; its genome is very complex , now known 6 genotypes and 100 species subgenomic type, these genotypes homology less than 65% , but these genotypes of the 5'- non - translated region of homology is greater than 85% , wherein the internal ribosome entry site (Internal RibosomeEntry Site IRES) sequence almost completely conserved . IRES is located in the mRNA of the 5'- untranslated region (5'-UTR), it can start does not depend on the start of the cap- structure of viral translation . Now known that the primary structure and secondary structure of the IRES , and know its primary structure and secondary structure of the HCV translation initiation is very important . Although its tertiary structure and related functions are not completely known , but already know its tertiary structure formation and ion concentration, related to the temperature and concentration of RNA . IRES comprises 390 nucleotides, by a series of connection points ( junctions ) , the ring ( 100 ps) and stems (stems) , can be broadly divided into four domains , and two mutually independent structure group . The IRES tertiary structure may be formed by the interaction of these domains . People have the tertiary structure of the IRES using the frozen electron microscopy , crystallization and X - diffraction and nuclear magnetic resonance method done some research, but these methods have their own limitations , and sometimes inconsistent or even contradictory results this is likely to be the IRES in the process of formation of the tertiary structure of its spatial conformation is constantly changing . This topic first design experiment, the use of of HCV IRES of the relevant sequence , synthesized and purified design of the DNA sequence , the in vitro transcription France RNA sequence and purification of this RNA sequence , go to phosphorylation of all RNA sequences and labeled portion of RNA sequence , and then studied at the different ion concentrations , different RNA concentrations and under different temperature conditions , the IRES structural domain ⅲ ( domain ⅲ ) of the connection point (iunction) at different positions on the structural domain ⅲ formed the influence of different sizes structure domain ⅱ complete domains III and incomplete domain III interaction and domain III of the connection points in the point mutation of the domain III formed .

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CLC: > Medicine, health > Basic Medical > Medical Microbiology ( pathogenic bacteriology,pathogenic microbiology ) > Human Virology ( pathogenic virus)
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