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Nitric oxide (NO) is a radical of the body of a simple structure. It is involved in a series of in vivo physiological activity, as well as participate in a variety of pathological processes, including cancer, including. Studies have shown that NO has a dual role on tumor growth. The one hand, NO can be formed by promoting tumor blood vessels and maintain the dilation of blood vessels in the tumor tissue to ensure maximum tumor blood supply, promote tumor growth; On the other hand, the high concentration of NO can kill tumor cells directly. Generated in vivo, NO is catalyzed by nitric oxide synthase (NOS), L-arginine. There are three subtypes of in vivo NOS, inducible (iNOS), neuronal (nNOS), endothelial (eNOS). The latter two are also known as structured (cNOS), the cell itself inherent has ca - calmodulin-dependent, less the amount of NO produced. iNOS, also known as Ca -independent, bacterial lipopolysaccharide, white interleukin-1 beta, gamma-interferon and other cytokines induced a relatively large amount of NO produced. In a variety of human solid tumors and tumor cell lines, NOS activity, especially iNOS activity was significantly higher, such as prostate cancer, breast cancer, gynecologic oncology. In breast cancer, gynecological cancer, NOS expression and tumor grade, prompt NOS may be involved in tumor progression, but the NOS and NO on the biological behavior of tumor mechanism is not yet fully understood. In bladder cancer is the most common malignancy of the genitourinary system, of which more than 90% of transitional cell carcinoma. As solid tumors, infiltration of the bladder tumor growth and metastasis D Zhejiang University graduate thesis DL vascular dependence diameter of solid tumors in vivo over L-Zmm when almost have a new supply of blood ll tube. Microvessel density (MVD) quantitatively reflect tumor angiogenesis. L in recent years, studies have reported NOS and NO and angiogenesis closely related. But in tumor bladder delay, D reported. We determined the transitional cell carcinoma of bladder skin NOS the three isoforms DL with microvessel density to study the NOS in bladder transitional cell carcinoma angiogenesis and hair LD students development. D 35 Delays in cases of bladder transitional cell carcinoma specimens were obtained from April 2000 to July 2000 the Zhejiang Medical DL hospital surgical specimens, including 27 males and female cases Lu; adjacent mucosa specimens in 12 cases, pathological card l is not real tumor invasion; the normal bladder umbilical mucosa specimens. Tumor tissue samples histopathological D stage: G18 cases, the GZ13 cases, G314 cases; Ti. ; 13 cases, T 12, T. _ * Example. The specimens were fixed in 10% formalin, embedded in paraffin and 5 pm serial sections. All subscript l The application SABC immunohistochemical method were performed nNOS, iNOS, eNOS and CD34 staining l color observed nitric oxide synthase (NOS) expression in transitional cell carcinoma of the bladder balcony and DIMVD correlation between sex. l11. expression of nNOS in: 35 tumor samples positive expression in 26 cases, the positive rate of 1 74.3%; 12 cases of adjacent mucosa specimens were positive in 10 cases; 8 normal bladder delay the mucosal specimens Dl positive in 4 cases. Among the three groups nNOS expression was no significant difference (PTH knife iNOS expression ll (see Table *: 35 tumor samples positive expression of the 30 cases, the positive rate of 85.7%: 121 cases willing NAO straw membrane specimen positive 8 cases; Lu the normal bladder umbilical mucosal specimens positive in 4 cases, groups of three Dl expression of iNOS statistically significant (PRTO, 05 * transitional cell carcinoma of iNOS expression compared with normal bladder mucosa, the difference was significant (Pwto.05L but bladder delay the transitional D cell carcinoma and adjacent mucosa, adjacent mucosa and normal bladder mucosa between the expression of iNOS D statistically significant. eNOS expression case: 35 tumor samples positive expression in 15 cases, yang DLFF $ 42.9%; IZ cases adjacent mucosa specimens were found in 7; Lu the normal bladder umbilical mucosa specimens LD D l Two main thesis of Zhejiang University Master of 1 positive in 3 cases among the three groups eNOS expression no significant difference (PTH 0.05) tumor tissue l nNOS, iNOS, eNOS expression and tumor grading, staging World statistically significant (Ptro.05) Table 1 transitional cell carcinoma of the adjacent mucosa, normal mucosal expression of iNOS in D __ mos expression of the upper world number __ 1 group number of cases 1111112222 on two hundred twenty-two thousand two hundred twenty-two L 2222 \'P I --- \gi, l 10.36 Guests 2.73,9.00 who: OI swollen ex group of MVD group, compared with the adjacent normal group adjacent mucosa, normal bladder mucosa MVD value for ZI * 2 Shilv. MVD count is high, invasive tumors (L) and poor in 1 do not have significant (P t 0.05) and high levels of tumor tissue (G) MVD count lower level 7 (G + G) farewell dinner table (T; _) I to high significant difference (Prto.05). the l 3.35 cases of bladder dirty transitional cell carcinoma
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