Dissertation > Excellent graduate degree dissertation topics show

Small ear pig cardiovascular system antigenic xenotransplantation research

Author: ZhangWei
Tutor: YingDaJun
School: Third Military Medical University
Course: Human Anatomy
Keywords: the inbred-line Banna pig cardiovascular system xenoantigen immunohistochemistry
CLC: R392.4
Type: Master's thesis
Year: 2001
Downloads: 33
Quote: 2
Read: Download Dissertation

Abstract


The xenoantigenicity of cardiovascular system on the inbred-line Banna pigAbstractOBJECT : The morphologic character and the distribution of xenoantigen (a -Gal ) on cardiovascular system of the Banna pig are observed and messured so as to provide information for xenotransplantation and breeding transgens pig.METHODS:The cardiovascular system on five Banna pigs (12-18 months old ) were studied and determined by gross anatomy, histology, affinity immuno-histochemistry and image analysis.RESULTS:(1) Banna pig heart and the human heart had many similarities in size, morphology and histology. However, the following differences were observed compared with the human heart: ①azygos vein directly drew into right atrium. ②The intercalated disk of cardiac muscle on Banna pig was less than the human. ③The Purkinje’s fibre on Banna big was less and bigger than the human. (2) The a -Gal positive staining was appeared in endothelia cells on endocardium, epicardium, connective tissue between cardiac muscles, membrane of nerve bundle, and so on. However, the cardiac muscles and nerve fibre were negative. The a -Gal positive staining was no significant difference between right and left atrium endocardium. The a -Gal positive staining was no significant difference between right and left ventricle endocardium. The a -Gal positive staining was no significant difference between epicardium and connective tissue between cardiac muscles. The a -Gal positive staining on atrium endoca2rdium was higher than ventricle endocardium with significant difference (P<O.O 1). The a -Gal positive staining on endocardium was higher than epicardium and connective tissue between cardiac muscles with significant difference (P<O.O 1). The a -Gal positive staining on cardiac valves(mitral valve)was higher than endocardium, epicardium and connective tissue between cardiac muscles with significant difference(P<O.O 1). (3) The a -Gal positive staining was appeared in endothelia cell and ectoblast on artery. However, smooth muscle cell, elastic fibre and collagenous fibre were negative.The thinner artery was, the stronger expression of a -Gal was on the artery endothelia cell(P<O.O 1). It is significant difference among four groups by T test(P<O.O I).CONCLUSSIONS:(1) On the morphology and histology, the heart of Banna pig is fit for pig-human heart xenotransplantation. (2) It is the difference that a -Gal expressed on cardiovascular system of the Banna pig. We can overcome HAR by breeding transgens pig. (3) The thrombus on micrangium is the important link on HAR. The prevetion and cure embolism in micrangium is key to resolve HAR on xenotransplantation.

Related Dissertations

  1. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  2. The Study of Kisspeptin/GPR54 System on the Reproductive Regulation in the Hen’s Hypothalamus-Pituitary-Ovary Axis,S831
  3. Study on the Digestive Tube During the Embryonic Stage of Gushi Chicken,S831
  4. A Comparative Study of prawns, crabs, Spiroplasma disease immunohistochemistry and pathology,S945
  5. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  6. Expressions of PRL-3 and p27 in Gastric Carcinoma and Their Clinical Significance,R735.2
  7. Expression of hnRNP A2/B1 and p21WAF1 Proteins in Lung Cancer and Their Clinical Significance,R734.2
  8. The Effects of RIP on the Immune Function of Renewal Rats Without Colostrums,S853.7
  9. Study on the Expression and Correlation of Metallothionein and Matrix Metalloproteinase-2 in Non-small Cell Lung Cancer,R734.2
  10. Expression and Their Significance of Apoptosis Regulatory Factors Survivin、XIAP and c-Jun、NF-κB in Epithelial Neoplasm of Ovary,R737.31
  11. Expression and Clinical Significance of Survivin and YAP in Cervical Carcinoma,R737.33
  12. Expression and Clinical Significance of PTEN and Caspase-7 in Gastric Cancer and Precancerous Lesion,R735.2
  13. Cloning and Expression Analysis of NCCRP-1 AND IL-10 Genes in Grass Carp (Ctenopharyngodon Idellus),S917.4
  14. Expression and Antibody Preparation of sglt1 from Cyprinus CarpioL.,S917.4
  15. The Effect of Hepatic Denervation on Liver Regeneration after Partially Hepatectomy in Rats,R363
  16. Expression and Correlation of Caspase-3 and Cx43 in Myocardial Infarction with Different Narrow Degree Coronary Artery,R542.22
  17. The Expression and Relationships of Bmi-1 and c-myc in Diffuse Large B-cell Lymphoma Tissues,R733.1
  18. Expression of Caspase-4 and Caspase-3 Gene Related Apoptosis in Pathological Changes of Sternocleidomastoid of Congenital Muscular Torticollis and Four Years Clinical Followed-up for Opereated Patients,R726.8
  19. The Feasibility of the Immunohistochemistry Classification to Predicting Prognosis in Breast Cancer,R737.9
  20. The Relationship Studying of Phosphatidylinositol-3-protein Kinase, Survivin Expression and Clinicopathologic of Rectal Carcinoma,R735.37
  21. Expression of NRP-2 and VEGF in Colorectal Adenocarcinoma and Colorectal Adenoma Tissues,R735.34

CLC: > Medicine, health > Basic Medical > Medical Immunology > Transplantation immunology
© 2012 www.DissertationTopic.Net  Mobile