|
Background: Diabetes (Diabetes mellitus, DM) is a systemic metabolic disorders that can lead to many organs, blood vessels and peripheral nerve lesions occur. Erectile dysfunction (Erectile dysfunction, ED) is common complication of diabetes, the incidence rate of 35-90%. Current first-line treatment of ED drugs in clinical phosphodiesterase type 5 inhibitors (Phosphodiesterase type 5 inhibitor, PDE5i), but not for all ED effective, PDE5i treatment of diabetic erectile dysfunction (Diabetes mellitus erectile dysfunction, DMED) have efficiency is only 50% or so, and there are some adverse reactions. Thus, still need to DMED etiology and pathogenesis and treatment be further explored. Endothelium-dependent hyperpolarizing factor (Endothelium-derived hyperpolarizing factor, EDHF) is an important vasodilator, SK3 (Small conductance calcium-activated potassium channels, SK3) as EDHF pathway critical substances in the process of penile erection important role. Study found that high blood pressure, diabetes and other diseases can affect EDHF pathway vasodilator effect, and high blood pressure can reduce the SK3 expression, but DM penis EDHF pathway mechanism is unclear. Objective: To study diabetic rat corpus cavernosum small conductance calcium-activated potassium channel protein expression SK3 explore the impact of DM on the penis EDHF pathway as well as the possible mechanism of SK3 expression. Methods: Male SD rats 60, 50 using a single intraperitoneal injection of streptozotocin (Streptozocin, STZ) 60mg/kg established diabetic model group (DM), rats were injected into a mold used STZ STZ failed to control group ( STZ), the remaining 10 for the control group (NDM). After the modeling eight weeks, subcutaneous apomorphine (Apomorphine, APO) 80μg/kg observed after penile erections, using RT-PCR, Western blot detection of SK3 mRNA and protein in rat corpus cavernosum expression. Results: 35 modeling success, raising eight weeks after 9 deaths. STZ rats were used as model drugs unpaired control group (STZ, n = 15), STZ group and NDM (NDM, n = 10) were no deaths. Erectile function tests found: DM group (n = 26) with 14 rat penile erection, erection was 54%, STZ group (n = 15) and NDM (n = 10) group number of animals and the erection erectile rates is 100%. SK3 mRNA expression in the DM group (0.50 ± 0.09) was significantly lower than the STZ group (1.15 ± 0.03) and NDM group (1.21 ± 0.04) (P lt; 0.05). SK3 protein expression in the DM group (0.65 ± 0.06) and STZ group (1.28 ± 0.039) and NDM group (1.34 ± 0.047) were significantly different when compared (P lt; 0.05). STZ group and between groups of rats with erectile NDM circumstances and SK3 mRNA and protein expression was no difference (P gt; 0.05). Conclusion: Diabetes can significantly reduce erectile function in rats, which may lead to the DM SK3 rat corpus cavernosum tissue decreased expression are closely related.
|