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The Research about Feridex Labeled MSCs Imaging Tracer in 3.0T Magnetic Resonance in Vitro

Author: ZhuZuoCheng
Tutor: TanJianMing
School: Fujian Medical
Course: Surgery
Keywords: Superparamagnetic iron oxide Mesenchymal stem cells Magnetically labeled cells Magnetic resonance imaging
CLC: R445.2
Type: Master's thesis
Year: 2010
Downloads: 14
Quote: 0
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Abstract


Objective: SD rat bone marrow mesenchymal stem cells (mesenchymal stem cells, MSCs) in vitro study of superparamagnetic iron oxide particles (superparamagnetic iron oxide particles, SPIO) labeled feasibility of SD rat bone marrow mesenchymal stem cells (mesenchymal stem cells, MSCs) biological activity . Of superparamagnetic iron oxide particles ( superparamagnetic iron oxide particles SPIO ) -labeled in vitro SD rat bone marrow mesenchymal stem cells (mesenchymal stem cells, MSCs) to cell imaging tracer feasibility 3.0T MR instrument . Method: 1 , isolated from SD rat bone marrow culture and identification of MSCs . 2 , at different concentrations in vitro the SPIO United polylysine mark , unlabeled cells as controls . Prussian blue staining and electron microscopy to identify the cells within the iron particles . Trypan blue staining to detect cell survival . 4, the magnetically-labeled MSCs transferred into fat osteogenic medium induced culture for later identification . 5 , Application 3.0T magnetic resonance instrument h2d1 1 54 the scan sequence tseBR2d1 1 6 scan sequence of magnetically labeled cells for imaging . Results: 1 , Prussian blue staining and electron microscopic examination showed that the cytoplasm contains dense iron particles . Trypan blue staining magnetically labeled MSCs activity no significant difference ( P gt ; 0.05 ) . The labeled cells can normally into fat osteoblast differentiation . 4, h2d1 1 54 the sequence and tseBR2d1 1 < / sub > 6 the sequence prompted compared to unlabeled cells signal intensity (SI) , 1 × 106/ml labeled cells , 5 × 105 / ml labeled cells SI were significantly decreased (P lt; 0.05), and signal strength attenuation rate of the two sequences the (△ SI) was significantly higher than unlabeled group ( P lt; 0.05 ) . 1 × 106/ml tag in two sequences the cell △ SI are higher than 5 × 105/ml labeled cells △ SI , but does not have a significant difference ( P GT ; 0.05) . CONCLUSION : SPIO in combination with poly - L - lysine can successfully labeled MSCs, magnetically labeled cell activity and the potential ability to differentiate . The magnetically labeled cells in the MR change of the characteristic of the low signal . Application 3.0TMR imaging tracer labeled cells viable tseBR2dl 1 6 sequence , h2d1 1 54 sequence imaging can obtain good results .

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CLC: > Medicine, health > Clinical > Diagnostics > Diagnostic Imaging > Magnetic resonance imaging
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