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The Change of Coagulation and Fibrinolysis Related Factors Expression in HUVECs Infected with Dengue Virus 2

Author: TangXiaoLong
Tutor: JiangZhenYou
School: Jinan University
Course: Pathogen Biology
Keywords: Dengue virus Umbilical vein endothelial cells Tissue factor Tissue factor inhibitor Thrombomodulin Tissue plasminogen activator Plasminogen activator inhibitor- 1 RT-PCR Dengue haemorrhagic fever Dengue shock syndrome
CLC: R373
Type: Master's thesis
Year: 2005
Downloads: 66
Quote: 0
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Abstract


Dengue virus type 2 - induced expression of vascular endothelial cells in blood coagulation and fibrinolysis associated molecular background and purpose of the research study : the major clinical manifestations of dengue hemorrhagic fever / dengue shock syndrome (Dengue haemorrhagic fever / Dengue shock syndrome, DHF / DSS) bleeding , increased vascular permeability and hypovolemic shock , suggesting that its pathogenesis may be involved in hemostasis defects . Vascular endothelial cells (vein endothelial cells, VECs) play an important role in the regulation of vascular permeability and maintain blood physiological state ( Dengue virus , DV ) dengue virus can infect people VECs DV VECs procoagulant and anticoagulant activity exists unclear. The VECs capable of synthesis and secretion of tissue factor ( tissue factor , TF) , tissue factor inhibitor (tissue factor inhibitor, TFPI), thrombomodulin (thrombomodulin, TM), tissue plasminogen activator (tissue plasminogen activator , tPA) and organization plasminogen activator inhibitor- 1 (tisslle plasrninogen activator inhibitor type-1, PAI-1) and other important molecules involved in the balance of the coagulation cascade and the fibrinolytic system . The VECs orderly synthesis of these molecules play a critical role in maintaining the blood physiological state . In the present study , Application DV 2 infection of human umbilical vein endothelial cells for ( human umbilica vein endothelial cells , HUVECs), observed the DV 2 HUVECs expression of tissue factor ( TF ) tissue factor inhibitor (TFPI) and thrombomodulin (TM) , and tissue plasminogen activator (tPA) and plasminogen activator inhibitor- 1 (PAI-1) and to explore the vascular endothelial cells in the role of DHF / DSS bleeding and plasma extravasation important pathophysiological process . Methods: trypsin digestion HUVECs were subcultured , and tested with the well-grown second, third generation cell . At the same time the application (cell counting Kit-8) CCK-8 DV 2 cell activity was measured before and after infection ; chromogenic substrate method for the determination of the cultivation of DV 2 and control groups supernatant tPA, PAI-1 activity ; reverse transcription polymerase chain reaction ( reverse transcript - polymerase chain reaction , RT - PCR ) method to detect cell TF , TFPI TM of tPA and PAI - 1 mRNA levels .

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CLC: > Medicine, health > Basic Medical > Medical Microbiology ( pathogenic bacteriology,pathogenic microbiology ) > Human Virology ( pathogenic virus)
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