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Glucose oxidase ( Glucose Oxidase EC1.1.3.4 GOD) under aerobic conditions catalyzed glucose produces gluconic acid and hydrogen peroxide , with removal of glucose , deoxy , sterilization , widely used in food , feed , medicine and other industries. Distributed in animals, plants and micro-organisms in vivo glucose oxidase , the main source of microorganisms but because of fast growth and reproduction , wide source of glucose oxidase . Currently, industrial production using mainly from Aspergillus niger or Penicillium the fermentation glucose oxidase . In this study, isolated and purified from the soil to get a glucose oxidase -producing bacteria identified by BIOLOG microbial identification system for Aspergillus niger ( Aspergillus niger ) , medium composition and physical and chemical factors on the production of glucose oxidase enzyme , to determine the optimum fermentation conditions . Through research , the following conclusions : the main nutrient medium (1 ) best ingredients were glucose , peptone , sodium nitrate . (2) the optimum concentration of the various nutrients in the culture medium were glucose 4 g / l 100g / l, peptone , sodium nitrate 3 g / l, hydrogen phosphate , dipotassium 2g / l of magnesium sulfate 0.7 g / l , calcium carbonate 3.5 g / l, KCl 0.5 g / l . The optimized medium production activity of 1.17μmol/min, and significantly improved than before optimization (0.79μmol/min) . ( 3 ) the aspergillus the Shaker fermentation of glucose oxidase 72h, the medium pH 6 , the culture temperature of 28 ° C , the oscillator speed of 200 rev / min , the inoculation amount to 10% , strain age is 36h. ( 4 ) of Aspergillus niger fermentor test conditions for the temperature 28 ° C , stirrer speed of 200 rev / min , pH 6 , fermentation time 48h . ( 5 ) bran solid-state fermentation fermentation of Aspergillus niger glucose oxidase optimal conditions : temperature 30 ℃, 72h, cycle liquid feed ratio of 1.5 .
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